Pre-made recombinant AAV; AAV-Synapsin-FLPo; AAV-Syn-FLPo; AAV5-Synapsin-FLPo; AAV5-Syn-FLPo
Product Category: Pre-made recombinant AAV; AAV-Synapsin-FLPo; AAV-Syn-FLPo;
AAV5-Synapsin-FLPo; AAV5-Syn-FLPo
Description: FLP
originally identified from Saccharomyces cerevisiae is a site-specific
recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT
sequence. It has been widely used for generating conditional knockout mice,
mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed
inefficient recombinase activity due to thermo-instability of the FLP protein.
Subsequent screening for thermo-stable mutants resulted in the identification of
FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the
recombination efficiency of FLPe in cells remains very low because of its
non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT
recombination efficiency for both in vivo and in vitro in mouse. This
AAV5-Synapsin-FLPo pre-made rAAV in serotype 5 (with capsid from AAV serotype 5
and 2xITR from AAV serotype 2) will over-express FLPo recombinase under human
synapsin promoter (exclusive neuronal expression). Ready to use format.
Titer:
> 1x1013 VG/mL
Size:
30
µL (Std Pack)
Storage Buffer:
PBS
Infection Protocol:
Vector Map:
