AAV-CaMKII-GCaMP6m (AAV Serotype 1)

Price: $488.00

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  • Catalog #: SL101473
  • Unit Size: 1 x >30 ÁL (>1E+13 VG/mL)

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Pre-made recombinant AAV; AAV-CaMKII-GCaMP6m; AAV1-CaMKII-GCaMP6m

Product Category: Pre-made recombinant AAV; AAV-CaMKII-GCaMP6m; AAV1-CaMKII-GCaMP6m

GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase.  When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms.

GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level.  Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast - GCaMP6m, slow - GCaMP6m and medium - GCaMP6m). 

The AAV1-CaMKII-GCaMP6m is a pre-packaged rAAV in serotype 1 (with capsid from AAV serotype 1 and 2xITR from AAV serotype 2) which over-expresses genetically encoded calcium indicator GCaMP6m under CaMKII promoter for neuron expression.   

Titer: > 1x1013 VG/mL

Size: > 30 ÁL (Std Pack)

Storage Buffer: PBS

Infection Protocol: 

Vector Map: 
Pre-made recombinant AAV; AAV-CaMKII-GCaMP6m; AAV1-CaMKII-GCaMP6m

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