Pre-made recombinant AAV; AAV-CaMKII-GCaMP6m; AAV1-CaMKII-GCaMP6m
Product Category:
Pre-made recombinant AAV; AAV-CaMKII-GCaMP6m;
AAV1-CaMKII-GCaMP6m
Description:
GCaMP is a
genetically encoded calcium indicator (GECI) and created from a fusion of green
fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from
myosin light chain kinase. When GECI binds
Ca2+, the conformational change of GCaMP induces proximity of the GFP parts,
eliciting strong GFP fluorescence signal, which allows measuring action
potentials and other receptor activation events that trigger Ca2+ influx. The
advantage of GECI's are that they can be genetically specified for studies in
living organisms.
GCaMP6
is a novel ultra-sensitive format of GCaMP that outperformed other sensors in
terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in
calmodulin part of GCaMP6 created several variants which showed different
calcium fluorescence decay rate (fast - GCaMP6m, slow - GCaMP6m and medium -
GCaMP6m).
The AAV1-CaMKII-GCaMP6m
is a pre-packaged rAAV in serotype 1 (with capsid from AAV serotype 1 and 2xITR
from AAV serotype 2) which over-expresses genetically encoded calcium
indicator GCaMP6m under CaMKII promoter for neuron expression.
Titer: >
1x1013 VG/mL
Size:
30 µL
(Std Pack)
Storage Buffer: PBS
Infection Protocol: 
Vector Map:
