Pre-made recombinant AAV; AAV-CAG-FLPo; AAV-CBA-FLPo; AAV8-CAG-FLPo; AAV8-CBA-FLPo
Product Category:
Pre-made recombinant
AAV; AAV-CAG-FLPo; AAV-CBA-FLPo; AAV8-CAG-FLPo; AAV8-CBA-FLPo
Description: FLP
originally identified from Saccharomyces cerevisiae is a site-specific
recombinases (SSR) from the λ integrase family and recognizes distinct 34 bp FRT
sequence. It has been widely used for generating conditional knockout mice,
mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed
inefficient recombinase activity due to thermo-instability of the FLP protein.
Subsequent screening for thermo-stable mutants resulted in the identification of
FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the
recombination efficiency of FLPe in cells remains very low because of its
non-mammalian origin.
FLPo is a mouse codon optimized FLPe that greatly increases the FRT
recombination efficiency for both in vivo and in vitro in mouse. This
AAV8-CAG-FLPo
pre-made rAAV in serotype 8 will express
FLPo recombinase under CAG promoter (also known as
CBA promoter).
Ready to use format.
Titer:
> 1x1013 VG/mL
Size:
>
30 µL (Std Pack)
Storage Buffer:
PBS
Infection Protocol:
Vector Map:
