How to eliminate toxicity of PepMute™, GenMute™ and PepMute™ Plus siRNA Reagents

PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium.  Using other cell culture mediums (supplemented with 10% FBS and antibiotics) to grow your cells will basically eliminate the transfection toxicity. For instance, if you find toxicity and high cell death, you are probably using DMEM base medium supplemented with 10% FBS and antibiotics to grow your cells.  To remove the transfection toxicity, you can change DMEM to DMEM/F12 or RPMI 1640 as base medium.   So far we have not figured out why, but it does minimize the toxicity.  Try it out!