shRNA/miRNA/gRNA Adenovirus Production Full Service Large Scale


  • Catalog #: SL100782
  • Unit Size: 2.0 mL at 5E+10~5E+11 PFU/mL

shRNA/miRNA/gRNA Adenovirus Production Full Service Large Scale

Production of Recombinant Serotype 5 shRNA/miRNA/gRNA Adenovirus (E1/E3 deletion) with Ad.MAX System:
- One-stop service: from shRNA/miRNA design to packaging.
- Unsurpassable transduction efficiency.
- Quick turnaround time: 3 to 4 weeks.
- Cost-effective.
- Special design request acceptable, FREE!


Large shRNA/miRNA/gRNA Ad.MAX™ shuttle vector collection for cloning shuttle plasmid with a specific promoter and reporter:

 Single-promoter Ad.MAX™ shuttle vector

 Dual-promoter Ad.MAX™ shuttle vector

      Promoterless
      CMV
      CAG
      H1
     
U6
      Synapsin
      UBC

      EF1α
      ALB(1.4)
      ApoE/AAT1
      CaMKII
      ELA1
      Enh358MCK
      cTNT
      GFAP
      MBP
      SST
      TBG

      αMHC
      hRPE
      mIP1
      tMCK



















 

      Promoterless
      CMV
      CAG
      H1
     
U6
      Synapsin
      UBC

      EF1α
      ALB(1.4)
      ApoE/AAT1
      CaMKII
      ELA1
      Enh358MCK
      cTNT
      GFAP
      MBP
      SST
      TBG

      αMHC
      hRPE
      mIP1
      tMCK

CMV
CAG
H1

U6
Synapsin
UBC

EF1α
CaMKII
cTNT
GFAP











 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 


Service Description:
1. Design, synthesize and clone shRNA/miRNA/gRNA to a shuttle vector followed by transfer of the whole transgene cassette to adenoviral genome.
2. Initial adenoviral stock production.
3. Adenovirus amplification from
~4000 cm2 Ad.MAX™ 293 cells.
4. Adenovirus purification via 2xCsCl ultracentrifugation.
5. Recombinant adenovirus PFU titration.


Required Materials: NCBI accession # of target gene, target region sequence, shRNA or siRNA sequence validated/published or mature miRNA sequence, miRNA accession # or gRNA sequence etc.

Turnaround Time: 3 ~ 4 weeks.

Deliverables: 2 mL (10x0.2 mL) of super purified in vivo grade adenovirus vector at 5E+10~5E+11 PFU/mL*.

We offer discount for new customer, please request a quote with us today.

We also offer truncated shRNA/miRNA/gRNA service, please
request a quote with us today.


* Final adenovirus yield may depend on the nature of transgene.



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