Description
By utilizing our innovative and proprietary lipid conjugation technology,
LipoD293™ (Ver. II) is specially designed and formulated by adding proprietary
enhancers for transfecting HEK293 cells and other mammalian cells (Figure 1). As a 2nd
generation liposome based DNA transfection reagent, LipoD293™ (Ver. II) offers
extremely high transfection efficiencies for HEK293 related cells as well as
many mammalian cells with less cytotoxicity. LipoD293™ reagent (Ver. II),
upgraded from its previous version with refined chemistry, is 3~4 times more
efficient in gene delivery. LipoD293™ reagent (Ver. II), 1.0 ml, is sufficient
for ~666 transfections in 24 well plates or ~333 transfections in 6
well plates.
Figure 1. A Cartoon Showing LipoD293™ (Ver. II) Enhanced Gene Delivery
Features
- Top choice for hard-to-transfect cells
- Exceptional high titers of virus production
- Equally good for very long DNAs (up to 180 kb)
- Equally good for suspension 293 cells (e.g., 293F, 293H, etc)
- High levels of recombinant protein production
- Presence of serum and antibiotics enhances efficiency on 293 cells
- Exceptional efficiency for both single DNA transfection and multi DNAs
co-transfection
- Very affordable
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Comparisons of Transfection
Efficiency of LipoD293™ DNA In Vitro Transfection Reagent (Ver. II) with Brand
Name Products
Comparison of transfection efficiency of LipoD293™ reagent (Ver. II) vs.
lipofectamine 2000 (L2K) and Fugene HD on HepG2 cells.
Right Panel: Comparison of transfection efficiency of LipoD293 (Ver. II) with
Lipofecatmine 2000 (L2K), and Fugene HD on HepG2 cells. GFP DNA (pEGFP-N3) was
transfected with different transfection reagents per manufacturer's protocols to
HepG2 cell (cultured on Collagen pretreated dishes). GFP positive cell (%) and
fluorescence intensity were detected by passing through FACS 48 hours post
transfection
Left Panel: presence of serum and antibiotics enhances LipoD293 (Ver. II)
efficiency on HepG2 cells. HepG2 cell (grown on collagen treated dishes) was
transfected with three different conditions-------serum and antibiotics free,
presence of 10% serum and antibiotics followed by removal 5 hours post
transfection and presence of 10% serum and antibiotics without removal 5 hours
post transfection.
Comparison of transfection efficiency of LipoD293™ reagent (Ver. II) vs.
lipofectamine 2000 (L2K), TransIT and Fugene 6 on CHO cells.
Right Panel: Comparison of transfection efficiency of LipoD293 (Ver. II)
with Lipofecatmine 2000 (L2K), TransIT and Fugene 6 on CHO cells. DNAs encoding
Renilla luciferase (phRL-CMV) and GFP (pEGFP-N3) were transfected with different
DNA transfection reagent per manufacturer's protocols. Renilla luciferase
activity and GFP fluorescence were detected with Renilla Assay System and a
Nikon Eclipse fluorescent microcopy respectively 24 hours post transfection.
Left Panel: Comparison of price ($/1.0 ml vial) of LipoD293 versus those
of Lipofecatmine 2000 (L2K), TransIT and Fugene 6. All the prices were collected
from the manufacturers' websites.
A comparison of transfection efficiency of LipoD293™ reagent with
lipofectamine 2000 (L2K) on a hard-to-transfect cell, primary rat aortic smooth
muscle cells. The rat aortic smooth muscle cells were prepared and
transfected with pEGFP-N3 by LipoD293™ reagent (left panel) and Lipofecatmine
2000 (L2K, right panel) respectively per manufacturers' protocols. The
transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon
Eclipse 2000 microscopy 24 hours post transfection. The above pictures were
kindly provided by Dr. Nickolai Dulin of Section of Pulmonary and Critical Care,
University of Chicago.
A comparison of transfection efficiency of LipoD293™ reagent with Fugene HD
on hard-to-transfect cell, LNCap cells. The LNCap cells were grown as ATCC
recommended procedures and co-transfected with pBabe-hygro-SSeCKs (1.5ug) and
pEGFP-N3 (0.5ug) per well (6 well plate) LipoD293™ reagent (left panel) and
Fugene HD (right panel) respectively per manufacturers' protocols. The
transfection efficiency was evaluated by detecting GFP fluorescence with a Nikon
Eclipse 2000 microscopy 24 hours post transfection. The above pictures were
kindly provided by Dr. Lyn Gao of Roswell Park Cancer Institute.
Two examples showing exceptional efficiency of LipoD293™ reagent on
hard-to-transfect cells like HepG2 and SaoS-2 cells. HepG2 and SaoS-2 cells
in 95% confluency were transfected with pEGFP-N3 and pSV-β-galactosidase DNAs
respectively in presence of serum/antibiotics. The efficiency was checked 48
hours post transfection by Zeiss 510 Confocal Microscopy and β-galactosidase
staining kit respectively.
A comparison of transfection efficiency of LipoD293™ reagent with 293fectin
on HEK293 cells. HEK293 cells transfected with pEGFP-C1 plasmid using
LipoD293™ In Vitro DNA Transfection Reagent (Ver. II) (upper panel) and the most
popular brand product 293fectin™ of Invitrogen (lower panel). The cells were
visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left
panel) and FITC imaging (right panel) 24 hours post-transfection.
A comparison of LipoD293™ (Ver. II) and Lipofecatmine LTX (LTX) on generation
of Lentivirus (LV). Three cDNAs were co-transfected with LipoD293™ (Ver. II)
and Lipofectamine LTX (LTX) into 293FT cells. A GFP vector, pHR-SIN-cppt-CMVEWP,
was used to determine titer of LV. 1x105 293F cells per well was plated in to a
24 well plate followed by addition of different of amounts of the vector
supernatant, 1 microliter (upper panel) and 10 microliters (lower panel)
respectively. 5 days later, the cells was passed with FACS. The numbers at the
upper right corner indicate the percentage of transduced cells. The titers of LV
generated with LipoD293™ and L2K were quantified to be 8x10^6 and 3x10^6 tu/ml
respectively
Technical Information & Datasheet
- LipoD293™ (Ver. II)
Reagent General Protocol & Data Sheet
- LipoD293™ (Ver.
II) Reagent for Lentivirus Production
To request a free trial sample, please
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shipping address and email us at
order@signagen.com
Testimonials
I am absolutely thrilled with the LipoD293 transfection reagent! I compared
LipoD293 to Lipofectamine for transfection of plasmids to generate viral
pseudoparticles and WOW! I recovered 4 times more virus using the LipoD293
reagent than Lipofectamine!! This is wonderful news for me and my mentor as it
means that I don't have to spend so much time (and resources) on transfecting
293T cells to recover virus for in vitro experiments. My mentor was also very
happy that LipoD293 costs much less than Lipofectamine!! We have already ordered
5 mls of LipoD293 and I have now convinced my lab mates to switch to LipoD293
since I got such good results with it. Thanks for making such a great product!
-------- Christy Lavine, Ph.D., Harvard University
I wanted to update you on the free sample of LipoD293 that you sent to us. I
recently transfected some Plat-E's side-by-side with Lipofectamine 2000, and
your product out-performed it!!! We are also validating it with HeLa cells, but
otherwise we are VERY happy with what we have seen thus far! Thank you for
sending us the free sample, it definately made the SALE for us!
--------Catherine Gallo, Ph.D., University of Cincinnati
we have now tried the LipoD293 DNA In Vitro transfection reagent on 293FT cells.
In the first flask we used the protocol by Invitrogen provided in theVirapower
box insert (Virapower, pBABE-GFP plasmid, plus Lipofectamine). In the second
flask we used LipoD293 (30 ul/6ml media) with the same amounts of Virapower and
plasmid as in the first flask. The results were stunning:LipoD293 gave twice as
many transformants as Lipofectamine 2000...
-------- A Beta Tester from Wayne State University
