HapiCell™ Transfection Toxicity Removal Cocktail

Price: $138.00

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  • Catalog #: SL100496
  • Unit Size: 0.125 liter

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Description:
HapiCell™ Transfection Toxicity Removal Cocktail was our proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity.  HapiCell™ Transfection Toxicity Removal Cocktail was developed to contain a mixture of several chemicals which were confirmed to effectively remove DNA/siRNA transfection mediated cytotoxicity.    Incubation of sensitive cells (e.g., SaoS-2, HUVEC, primary kerotinocytes, etc) with HapiCell Toxicity Removal Reagent for 2 minutes at RT was confirm to completely remove the transfection related toxicity which otherwise kill the cells.  The cocktail was used for chemical transfection only, not for electroporation.  HapiCell™ Transfection Toxicity Removal Cocktail, 125 ml, is sufficient to treat 125 wells for 24-well plate and 63 wells for 6-well plate respectively.

Feature:
- Removes transfection mediated toxicity after 2 minutes incubation at RT
- Nontoxic to cells
- Maintain cell viability
- Improve transfection efficiency
- Easy to use

HapiCell™ Transfection Toxicity Removal Cocktail Effectively Remove Transfection Mediated Toxocity:

A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on mouse skin fibroblast. A DNA encoding GFP was delivered to mouse skin fibroblasts by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (right panel) or without (left panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection


A example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on primary mouse kerotinocytes. A DNA encoding GFP was delivered to primary mouse kerotinocytes by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (right panel) or without (left panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection

Data Sheet



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