GenJet™ In Vitro DNA Transfection Reagent for 3LL Cells

Price: $156.00

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  • Catalog #: SL100489-3LL
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for 3LL cell is pre-optimized for transfecting 3LL cells. 3LL also called Lewis lung carcinoma was discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass.

Refer to the following optimal transfection conditions for maximal transfection efficiency on 3LL cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


80%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, 18~24 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter)

88%


Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on 3LL Cells. 3LL cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection

Data Sheet




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