GenJet™ In Vitro DNA Transfection Reagent for MDCK Cells

Price: $156.00

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  • Catalog #: SL100489-MDCK
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for MDCK cell is pre-optimized for transfecting MDCK cells. MDCK cell line is derived from the kidney of an apparently normal, adult, female cocker spaniel; used in numerous viral studies; susceptible to vesicular stomatitis, vaccinia, and Coxsackie viruses, and certain reoviruses and adenoviruses.

T
he "Shaved Cell Transfection" protocol was optimized to achieve maximal transfection efficiency. Refer to the following optimal transfection conditions for maximal transfection efficiency on MDCK cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 transfections in 24 well plates or 150 transfections in 6 well plates.

Summary of Optimal Shaved CellTransfection Conditions:
Cell culture medium
Confluency of cell on the day of shaving
Cell density for plating

GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Incubation time of shaved cells with transfection complex
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


DMEM with 4.5 g/L glucose, 10% FBS

95~100%
1.3x10^5 cells per square c
entimeter
4:1
Serum-free DMEM with 4.5 g/L glucose

12 minutes at RT
20 minutes at 37 degree
48 hours


EGFP
pEGFP-N3 (CMV promoter)

70%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer


A Picture Showing Transfection Efficiency of GenJet™ Reagent on MDCK Cells. MDCK cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection

Data Sheet




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