GenJet™ In Vitro DNA Transfection Kit for Keratinocytes

Price: $168.00

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  • Catalog #: SL100489-KERA
  • Unit Size: 1 kit (2x1.0 ml)

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Contents Per Kit:
1x1.0 ml of GenJet™ DNA Transfection Reagent
● 1x1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail

Description

GenJet™ DNA In Vitro Transfection Reagent for keratinocytes is pre-optimized for transfecting human or mouse primary or immortalized keratinocytes. Keratinocytes are hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 50% efficiency with minimal cell death.

Refer to the following optimal transfection conditions for maximal transfection efficiency on
keratinocytes
. GenJet™ reagent, 1.0 ml, is sufficient for ~600 transfections in 24 well plates or ~300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Treatment with HapiCell™ Toxicity Removal Cocktail
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


90%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
Yes, ~5 Hours After Transfection Followed by PBS Wash
48 hours


EGFP
pEGFP-N3 (CMV promoter)

56%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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