GenJet™ In Vitro DNA Transfection Reagent for SHEP Cell

Price: $156.00

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  • Catalog #: SL100489-SHEP
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for SHEP is pre-optimized for transfecting SHEP cells. This human neuroblastoma cell line was cloned from a single primary tumor. Unlike other neuroblastoma cell, SHEP cells does not produce IGF-II, expresses five-fold fewer IGF-IRs than SH-SY5Y and dies in serum-free media or following exposure to metabolic stressors.

Refer to the following optimal transfection conditions for maximal transfection efficiency on SHEP
cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~85%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter)

80%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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