GenJet™ In Vitro DNA Transfection Kit for HUVEC

Price: $268.00

Add to Cart:

  • Catalog #: SL100489-HUVEC
  • Unit Size: 1 kit (2x1.0 ml)

Please Choose:



Contents Per Kit:
1x1.0 ml of GenJet™ DNA Transfection Reagent
● 1x8.0 ml (x5 ) of GenJetll™ Transfection Buffer

Description

GenJet™ DNA In Vitro Transfection Reagent for HUV
EC is pre-optimized for transfecting human umbilical vein endothelial cells (HUVEC).  HUVEC is one of hard-to-transfect cell lines partly due to the transfection toxicity related cell death. In combination of GenJet™ Transfection Buffer, the pre-optimized transfection reagent gave up to 60% efficiency. Refer to the following optimal transfection conditions for maximal transfection efficiency on HUVEC. GenJet™ reagent, 1.0 ml, is sufficient for 500 transfections in 24 well plates or 250 transfections in 6 well plates.

Summary of Optimal Shaved CellTransfection Conditions:
Cell culture medium
Confluency of cell on the day of shaving
Cell density for plating

GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


DMEM with 4.5 g/L glucose, 10% FBS
70%
1.3x10^5 cells per square c
entimeter
2.25:1

GenJet™ Transfection Buffer (1x )
10 minutes at RT
48 hours


EGFP
pEGFP-N3 (CMV promoter)
56%

Storage Condition
Store at 4 0C. If stored properly, the product is stable for 12 months or longer

Data Sheet




Copyright © 2017 SignaGen Laboratories. All Rights Reserved.