GenJet™ In Vitro DNA Transfection Reagent for WEHI-231 Cells

Price: $298.00

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  • Catalog #: SL100489-WEHI
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for WEHI-231 is pre-optimized for transfecting WEHI-231 cells. WEHI-231 is a murine B cell lymphoma that has been used extensively as a model for the immature stage B cell and its functional response to Ag receptor cross-linking as a model for immature B cell tolerance. This cell line expresses sIgM, CD5, and FcR gamma, but lacks the B cell- specific isoform of CD45 (B220).

Refer to the following optimal transfection conditio
ns for maximal transfection efficiency on WEHI-231
cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~80%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
1x GenJet™ Transfection Working Buffer

10 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter)

66%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet




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