Skip to content

Miscellaneous - 13. page

mEos3.2

Monomeric variant of green-to-red photoswitchable fluorescent protein EosFP, engineered for reduced aggregation relative to mEos2. ATGAGTGCGATTAAGCCAGACATGAAGATCAAACTCCGTATGGAAGGCAACGTAAACGGGCACCACTTTGTGATCGACGGAGATGGTACAGGCAAGCC TTTTGAGGGAAAACAGAGTATGGATCTTGAAGTCAAAGAGGGCGGACCTCTGCCTTTTGCCTTTGATATCCTGACCACTGCATTCCATTACGGCAACAGG GTATTCGCCAAATATCCAGACAACATACAAGACTATTTTAAGCAGTCGTTTCCTAAGGGGTATTCGTGGGAACGAAGCTTGACTTTCGAAGACGGGGGC ATTTGCAACGCCAGAAACGACATAACAATGGAAGGGGACACTTTCTATAATAAAGTTCGATTTTATGGTACCAACTTTCCCGCCAATGGTCCAGTTATGC AGAAGAAGACGCTGAAATGGGAGCCCTCCACTGAGAAAATGTATGTGCGTGATGGAGTGCTGACGGGTGATATTGAGATGGCTTTGTTGCTTGAAGGA AATGCCCATTACCGATGTGACTTCAGAACTACTTACAAAGCTAAGGAGAAGGGTGTCAAGTTACCAGGCGCCCACTTTGTGGACCACTGCATTGAGATT TTAAGCCATGACAAAGATTACAACAAGGTTAAGCTGTATGAGCATGCTGTTGCTCATTCTGGATTGCCTGACAATGCCAGACGATAA

Transfection Techniques for Neuronal Cells

Summary of advantages and disadvantages of different techniques commonly used to transfect mammalian neurons Best suited for Strengths Limitations Toxicity Onset, level, and duration of expressiona Maximum insert size Genome […]

mIFP Full Sequence

atgtcggtaccgctgactacctcagcattcggccacgcgtttctggctaactgtgaacgcgagcagatccacctggcgggctccattcagccgcac ggtatcctgctggctgtgaaagagccggacaacgtggtgatccaggcttctattaacgctgcggagttcctgaacaccaactctgttgttggcc gtccgctgcgtgacctgggcggcgatctgcctttgcagatcctgccgcacctgaacggcccgctgcacctggctccgatgaccctgcgttgtacc gtgggttctccgccgcgtcgtgtggactgtaccattcatcgtccgtctaacggcggcctgatcgtagaactggaaccagcaaccaagaccact aacattgcgccggctctggacggtgcgtttcatcgtatcacttcttcatcctccctgatgggcctgtgtgacgaaaccgcgactattatccgtgaga ttactggctacgaccgtgtgatggtagtacgtttcgatgaagagggtaatggcgaaattctgtccgaacgtcgtcgtgcggacctggaagcgtt cctgggtaaccgctacccggcgtctactattccgcagatcgctcgtcgcctgtacgaacataaccgtgttcgcctgctggtagatgtgaactat actccggttccgctacagccgcgcatcagcccgctgaacggtcgtgatctggatatgtccctgtcttgcctgcgctctatgtccccgatccacca gaaatacatgcaggacatgggcgttggcgcgaccctggtttgctctctgatggtgtctggtcgtctgtggggtctgatcgcttgccaccacta cgaaccgcgcttcgttccgttccacattcgcgctgctggcgaagcgctggcggaaacttgtgcgatccgcatcgcgacgctggagagcttt gcacagtctcagtccaaatga

Infrared fluorescent proteins

Hunting for new fluorescent proteins in the coral reefs of the Caribbean and Australia is a task that a lucky few researchers have managed to get funding for. Scuba diving […]

NLSs

SV 40 Nucleoplasmin: AVKRPAATKKAGQAKKKKLD EGL-13: MSRRRKANPTKLSENAKKLAKEVEN c-Myc: PAAKRVKLD TUS-protein: KLKIKRPVK

Universal Primers

Standard primer name Sequence (5′->3′) Number of bases 1629DWN CTG TAA ATC AAC AAC GCA CAG 21 3AOX1 <GCA AAT GGC ATT CTG ACA TCC 21 5AOX1 < GAC TGG […]

Universal Primer List

  PRIMERS SEQUENCES 3’ AD 5’ (AGA TGG TGC ACG ATG CAC AG) 3’ 3 AOX1 5′ (GCA AAT GGC ATT CTG ACA TCC) 3’ 5’ AD 5’ (TTC GAT […]