CCAGAAATGGCGCCGGAGGCGGGAACAAGGTGGTGGATGAGTGCTACATCCCCAATTACTTGCTCCCCAAAACCCAGCCTGAGC TCCAGTGGGCGTGGACTAATATGGAACAGTATTTAAG
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p40 Promoter
GGGTCACCAAGCAGGAAGTCAAAGACTTTTTCCGGTGGGCAAAGGATCACGTGGTTGAGGTGGAGCATGAGTTCTACGTCAAAAAGGGTGGAGCCAAGAAAAG ACCCGCCCCCAGTGACGCAGATATAAGTGAGCCCAAACGGGTGCGCGAGTCAGTTGCGCAGCCATCGACGTCAGACGCT
TATAless p5 Promoter
GGAGGGGTGGAGTCGTGACGTGAATTACGTCATAGGGTTAGGGAGGTCCTGTATTAGAGGTCACGTGAGTGTTTTGCGACATTTTGCGACACCA TGTGGTCTCGCTGGGGGGGGGGGCCCGAGTGAGCACGCAGGGTCTCCATTTTGAAGCGGGAGGTTTGAACT
Validated Target Region against Mouse TIMP1 Gene
# 1. tgcacagtgtttccctgtt # 2. tcaagatgactaagatgct # 3. tgcacagtgtttccctgtt
Determining the concentration of protein in SDS-PAGE gel bands using ImageJ
Determining the concentration of protein in SDS-PAGE gel bands using ImageJ To determine protein concentration you will need to have a standard curve to compare your samples to – For […]
qPCR Primer and Probe Sequences for rAAV Titration
CMV Promoter: CMV-F TTC CTA CTT GGC AGT ACA TCT ACG CMV-R GTC AAT GGG GTG GAG ACT TGG CMV-P TGA GTC AAA CCG CTA TCC ACG CCC A AAV2 […]
Commonly Used Primers
3’AOX1 GCAAATGGCATTCTGACATCC For Pichia vectors with AOX1 terminator, reverse primer 5’AOX1 GACTGGTTCCAATTGACAAGC For Pichia vectors with AOX1 promoter, forward primer 35S promoter CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer AC5 ACACAAAGCCGCTCCATCAG […]
Sim1 Specific Promoter for PVN Neurons
1 GGTACCGAGG CGCGCAGCCG TGGGGAAATC CTGGCCACTG TCTGGGATGC 51 TATTCGCACG CGTTCCTGGA GCCTCGGCTA GCCAGCCCCC GCAAGGCCGC 101 ACGCTGTGCG CAGACACCCG AGTCTAGGGA GCGCGAGTGT CACTCGGCAC 151 GCCGAAGACC TCGGGGTTTG GAGCGGCGGC GGCAGCGGGG CAGCGGCGAC 201 CCCGCGCCCG GGGCTCATTA GGAGCGGAGC TGCCAGGCTC ACTGTAGGAC […]