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All posts by signagen

Gibson Assembly Cloning

Summary In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear DNA fragments (Nat Methods 2009;6(5):343-5). […]

Tips for Maximizing Ligation Efficiencies

T4 DNA Ligase is the most extensively used ligase for cloning-based experiments. Traditionally, a ligation reaction (blunt or cohesive ends) using traditional T4 DNA Ligase involves incubation at 16°C using […]

Troubles with ligation?

Check the activity of your T4 DNA Ligase with two easy-to-do control experiments You may check the efficiency of your ligation reaction by mixing the reaction with loading dye containing […]

Electroporation vs. Heat Shock Transformation

The advantages of using electroporation are the higher efficiency, more colonies, and much faster transformations compared to heat shock method. Transformation efficiencies for electroporation are 5.0 x 109 – 2.0 x […]