All posts by signagen
The Principle of Transformation
As DNA is a highly hydrophilic molecule, it usually cannot pass through the cell membrane of bacteria. Hence, to make bacteria capable of internalizing genetic material, they must be made […]
IRES or 2A in my polycistronic expression cassette, which one is better?
When designing a gene expression vector to co-express multiple ORFs under the control of a single promoter, you can choose to place multiple ORFs behind the promoter, separated by linkers […]
How Orbital Diameter and Shaker Agitation Rate Affect Bacteria Growth
A common use for orbital shakers is to grow bacteria for a variety of purposes, including the production of proteins and genetic matter and for the study of bacteria themselves. […]
Gibson Assembly Cloning
Summary In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute developed a novel method for the easy assembly of multiple linear DNA fragments (Nat Methods 2009;6(5):343-5). […]