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Lentivirus Formulation Buffer
20 mM PIPES, 75 mM NaCI, 2.5% sucrose, pH 6.520 mM HEPES, 75 mM NaCI, 2.5% sucrose, pH 7.0
A195 Buffer for Adenovirus Formulation and Long Term Storage
10 mM Tris-HCl, 10 mM histidine, 75 mM NaCl, 5% sucrose (w/v), 1 mM MgCl2, 0.02% (w/v) PS-80 (polysorbate-80), 0.1 mM EDTA, 0.5% Ethanol (v/v), pH 7.4
DNA/RNA non-specific endonuclease [Serratia marcescens]
atgcgctttaacaacaagatgttggccctggccgccctgctgttcgccgcacaggcgtcggccgacacgttcgaatccatcgacaactgcgcggtcggctgcccgaccggcggcagcagcaacgtgtcgatcgtgcgtcatgcttatacgttgaacaacaacagcacaaccaagttcgccaactgggtggcctatcacatcaccaaagacacgccggccagcggcaagacgcgcaactggaaaaccgacccggcgctcaatccggccgacaccctggcgcccgccgattacaccggcgccaacgcggcgctgaaggtcgatcgcggtcatcaggcgccgctggcctcgctggcgggcgtttccgactgggaatcgctgaactacctgtccaacatcacgccgcaaaagtccgatcttaaccagggcgcctgggcgcggctggaagatcaggaacgcaagctgatcgatcgcgccgacatctcctcggtctataccgtgaccgggccgttgtatgagcgtgatatgggcaaactgccgggcacccagaaagcgcacaccatccccagcgcctactggaaggtgattttcatcaacaacagcccggcggtaaaccactatgccgctttcctgttcgatcagaacacgccgaagggcgccgatttctgccaattccgcgtgacggtggacgagatcgagaaacgtaccggcctgatcatctgggccggtctgccggacgacgtacaggcttcgctgaagagcaagcccggcgtcctgccggagctgatgggctgcaaaaactga
DNA/RNA non-specific endonuclease [Serratia marcescens]
ATGCGCTTCTCCAACAAAATGCTTGCGTTGGCAGCACTGCTGTTTGCAGCGCAAGCGAGTGCAGATACCCTGGAGTCGATTGACAATTGTGCTGTGGGTTGTCCGACAGGAGGCAGCAGTAACGTGAGCATCGTACGTCATGCGTATACCCTGAACAACAATAGCACCACCAAATTTGCGAATTGGGTGGCGTACCATATTACGAAGGACACGCCGGCCTCTGGGAAAACCCGGAATTGGAAAACCGATCCCGCGCTGAATCCGGCCGATACTCTGGCACCAGCCGACTATACAGGCGCGAATGCTGCTCTGAAAGTGGATCGTGGGCATCAAGCCCCTTTAGCGTCACTTGCCGGCGTTTCTGACTGGGAATCGCTCAACTATCTCAGCAACATTACGCCGCAGAAATCTGACCTTAACCAGGGTGCATGGGCACGCTTGGAAGATCAGGAACGCAAGCTGATTGATCGTGCGGACATTTCATCCGTCTACACCGTTACTGGTCCGCTGTATGAACGCGATATGGGCAAATTACCGGGCACGCAGAAAGCGCACACGATTCCCAGTGCTTACTGGAAAGTGATCTTCATCAACAATTCGCCTGCGGTCAACCACTATGCTGCGTTCCTGTTCGATCAGAACACGCCAAAAGGTGCCGACTTTTGCCAGTTTCGCGTAACCGTAGATGAAATCGAGAAACGTACCGGCCTGATCATCTGGGCCGGTTTACCGGATGATGTGCAGGCATCCTTGAAAAGCAAACCGGGCGTTCTGCCAGAACTGATGGGTTGCAAGAATTAA
DNA/RNA non-specific endonuclease [Serratia marcescens]
1 MRFNNKMLAL AALLFAAQAS ADTLESIDNC AVGCPTGGSS NVSIVRHAYT LNNNSTTKFA 61 NWVAYHITKD TPASGKTRNW KTDPALNPAD TLAPADYTGA NAALKVDRGH QAPLASLAGV 121 SDWESLNYLS NITPQKSDLN QGAWARLEDQ ERKLIDRADI SSVYTVTGPL YERDMGKLPG 181 TQKAHTIPSA YWKVIFINNS PAVNHYAAFL FDQNTPKGAD FCQFRVTVDE IEKRTGLIIW 241 AGLPDDIQAS […]
Starting Materials to Run a ddPCR for rAAV Titration with An AutoDG Droplet Generator and QX 200 Droplet Reader
Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes no dUTP, Bio-Rad,1863023 10% Pluronic F-68, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, […]
Tips and tricks for performing ddPCR
Clean everything You must have a clean working area to titer your AAV by ddPCR. Droplet digital PCR is a sensitive assay, so cross contamination into a no template control […]
Adenovirus Type C qPCR Titration Primers and Probe
Target Penton:FORWARD: TCGACACCACCCGTGTGTACREVERSE: TGCTGTGGTCGTTCTGGTAGTTPROBE: TGGACAACAAGTCAACGGATGTGGCA