Research Use Only 2019-Novel Coronavirus (2019-nCoV, Covid-19) Real-time RT-PCR Primers and Probes

Name	Description	Oligonucleotide Sequence (5’>3’)	Label¹	Final Conc.
2019-nCoV_N1-F	2019-nCoV_N1 Forward Primer	GAC CCC AAA ATC AGC GAA AT	None	500nM
2019-nCoV_N1-R	2019-nCoV_N1 Reverse Primer	TCT GGT TAC TGC CAG TTG AAT CTG	None	500nM
2019-nCoV_N1-P	2019-nCoV_N1 Probe	FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1	FAM, BHQ-1	125nM
2019-nCoV_N1-P	2019-nCoV_N1 Probe	FAM-ACC CCG CAT /ZEN/ TAC GTT TGG TGG ACC-3IABkFQ	FAM, ZEN, 3IABkFQ	125nM
2019-nCoV_N2-F	2019-nCoV_N2 Forward Primer	TTA CAA ACA TTG GCC GCA AA	None	500nM
2019-nCoV_N2-R	2019-nCoV_N2 Reverse Primer	GCG CGA CAT TCC GAA GAA	None	500nM
2019-nCoV_N2-P	2019-nCoV_N2 Probe	FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1	FAM, BHQ-1	125nM
2019-nCoV_N2-P	2019-nCoV_N2 Probe	FAM-ACA ATT TGC /ZEN/ CCC CAG CGC TTC AG-3IABkF	FAM, ZEN, 3IABkFQ	125nM
RP-F	RNAse P Forward Primer	AGA TTT GGA CCT GCG AGC G	None	500nM
RP-R	RNAse P Reverse Primer	GAG CGG CTG TCT CCA CAA GT	None	500nM
RP-P	RNAse P Probe	FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1	FAM, BHQ-1	125nM
RP-P	RNAse P Probe	FAM-TTC TGA CCT /ZEN/ GAA GGC TCT GCG CG-3IABkFQ	FAM, ZEN, 3IABkFQ	125nM