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Monthly Archives: October 2015 - 8. page

WHAT MAKES LENTIVIRUS SO SPECIAL?

Though there are several virus delivery systems available today for gene therapy, lentivirus is considered one of the best methods.  Several features make lentivirus preferable especially the ability to infect […]

Overview of Lentivirus Pseudotypes

Family Genus Species Vector References Rhabdoviridae Vesiculovirus Vesicular stomatitis virus (Indiana virus) HIV-1 [Akkina, 1996][Naldini, 1996][Reiser, 1996] HIV-2 [Poeschla, 1998a] FIV [Poeschla, 1998b] EIAV [Olsen, 1998] BIV [Berkowitz, 2001a] JDV […]

rAAV Vector Tropism

rAAV Vector Tropism: rAAV Serotype Applications (X = Recommended for use) Skeletal muscle Heart Lung CNS / Eye Liver Adipose AAV1 X X X X – – AAV2 – – […]

Real Time PCR Dyes

Dye Structure Color Vendor Cat # Price Features SYBR Green 1 Green unstable at 4 °C SYTO-82 Orange does not inhibit PCR, show no preferential binding to GC rich sequences […]

GCaMP6 calcium indicators

Name Description Addgene Plasmid ID Reference CMV-GCaMP6s improved SNR, slower kinetics 40753 Chen et al., 2013 CMV-GCaMP6m improved SNR, intermediate kinetics 40754 Chen et al., 2013 CMV-GCaMP6f improved SNR, faster […]

Cre-inducible Adeno-associated Virus: DIO-AAV

For cell type-specific targeting and to capitalize on the large number of Cre driver lines with the flexible virus injection/fiberoptic approach, we have developed a tool we call DIO-AAV (doublefloxed […]

Primers for Lentivirus Titration using qRT-PCR

Vector and region amplified Primers and probes dedicated for the amplification of lentiviral or transgenic DNA sequences Primers and probes dedicated for the amplification of internal control DNAs (cellular genome […]