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Daily Archives: October 12, 2015

>100 Pre-made Ready to Use rAAVs from SignaGen

All the following pre-made AAVs were packaged in serotype 1**, 2, 5, 6, 8** and 9** and super purified via 2xCsCl ultra-centrifugation in in-vivo grade.  All the pre-packaged rAAVs were […]

Summary of clinical trials using AAV gene transfer

Disease Transgene product Serotype Route of administration Clinical trial ClinicalTrials.gov identifier Refs AAV clinical trials for inherited diseases α1 antitrypsin deficiency α1 antitrypsin AAV2 Intramuscular Phase I/II NCT00377416 101,102 AAV1 […]

Key properties of a viral vector

Viral vectors are tailored to their specific applications but generally share a few key properties: Safety: Although viral vectors are occasionally created from pathogenic viruses, they are modified in such […]

Pre-made Lentivirus from GeneCopoeia

Positive and negative controls: eGFP eYFP eCFP mCherry PLUM Firefly luciferase Renilla luciferase Gaussia luciferase Firefly luciferase + eGFP/mCherry Renilla luciferase + eGFP/mCherry Firefly luciferase + IRES-mCherry Renilla luciferase + […]

WHAT IS PSEUDOTYPING of LENTIVIRUS?

In HIV, pseudotyping is used to change the tropism of the virion.  In other words, pseudotyping changes the way the Lentivector targets a specific cell for infection.  In wild type […]

WHAT MAKES LENTIVIRUS SO SPECIAL?

Though there are several virus delivery systems available today for gene therapy, lentivirus is considered one of the best methods.  Several features make lentivirus preferable especially the ability to infect […]

Overview of Lentivirus Pseudotypes

Family Genus Species Vector References Rhabdoviridae Vesiculovirus Vesicular stomatitis virus (Indiana virus) HIV-1 [Akkina, 1996][Naldini, 1996][Reiser, 1996] HIV-2 [Poeschla, 1998a] FIV [Poeschla, 1998b] EIAV [Olsen, 1998] BIV [Berkowitz, 2001a] JDV […]