LipoJet™ In Vitro Transfection Kit (Ver. II)

Price: $288.00  $259.00
Save: 10% off

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  • Catalog #: SL100468
  • Unit Size: 1.0 ml

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Description:
Based on our innovative and proprietary lipid-conjugation technology, LipoJet™ Transfection Kit, formulated from novel fluorinated cationic lipids, exhibits significant difference from other lipids transfection reagents in the market. LipoJet™ Transfection Kit is the most powerful yet very gentle gene delivery tool for a variety of applications including plasmid DNA and/or siRNA for most of mammalian cell types.  Compared with leading products in the market, LipoJet™ is more cost-effective and always provides higher transfection efficiency with less cytotoxicity.

Kit content:
- LipoJet™ Reagent, 1.0 ml sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate).

- LipoJet™ Transfection Buffer (5x ), 8.0 ml to make 40 ml of working solution (1x ).

Features: 
- Versatile: DNA transfection, siRNA transfection and DNA/siRNA co-transfection.
- Powerful: 
Outstanding efficiency on adherent cells.
- Extremely low toxicity: Improve Cell viability after transfection
- Economical: More transfections with less reagent
- Easy to use: One tube reaction and compatible with serum/antibiotics.
- Suitable for high-throughput application


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Broad Transfection Spectrum for Mammalian Cell Types

Cell Line

DNA Transfection

siRNA Transfection

293, 293T
3T3, NIH 3T3
3T3-L1
A549
B16-F10
BNLCL.2
C2C12
C3H/10T1/2
Caco-2
Rat primary cardiomyocytes
CHO
COS1
COS7
Human bladder carcinomal cell
mES
iMEF
HBEC
HCT116
Hela
HepG2
HT-29
HuH-7
LNCaP
MC3T3-E1
MCF10A
MCF7
MDA-MB-231
MDCK
MEF
Human primary melanocytes
Human primary melanoma
Human immortalized microglial
mIMCD-3
MRC-5
N2A
PC3
RAW 264.7
RPE
SH-SY-5Y
SK-OV-3
U-2OS
VERO

85%
65%
30%
80%
75%
55%
80%
60%
20%
10%
80%
70%
75%
80%
50%
55%
45%
75%
80%
60%
45%
45%
45%
50%
40%
50%
50%
20%
50%
50%
50%
50%
50%
50%
68%
70%
45%
30%
75%
70%
80%
40%

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90% at 20 nM
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90% at 10 nM
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90% at 10 nM
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80 % at 20 nM
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85% at 30 nM
85% at 30 nM
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90% at 25 nM
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Examples Showing Excellent DNA and siRNA Transfection Efficiency of LipoJet™ In Vitro Transfection Kit
LipoJet Transfection Reagent
A efficiency comparison of LipoJet™ reagent vs. brand name products to transfect Hela, Cos-7, NIH-3T3, CHO and HEK293 (left panel) and Cos-7 (right panel).  pEGFP-N3 cDNA (0.5 µg/well of 24-well plate) was transfected into different mammalian cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The transfection efficiency were analyzed via FACS 48 hours post transfection.

LipoJet_Cell_Viability
A toxicity comparison of LipoJet™ reagent vs. brand name products to transfect Hela cells.  pEGFP-N3 cDNA (1.0 µg/well of 24-well plate) was transfected to Hela cells per the standard transfection protocols in presence of serum (10% FBS) and antibiotics as recommended by manufacturers.  The MTT assay (right panel) and phase contrast imaging (left panel) were used to analyze the cell viability 48 hours post transfection.

LipoJet_LIPO2000_POLYJET_Comparison_HEK293
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet_LIPO2000_POLYJET_Comparison_HELA
A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell.  pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet_GFP_siRNA_HEK293
LipoJet_GFP_siRNA_HELA
LipoJet_GFP_siRNA_SaoS-2
Exceptional gene silencing of LipoJet™ reagent during DNA/siRNA co-transfection on mammalian cells. Transfection of GFP cDNA (left panel, 0.25 µg per well of 24-well plate) and co-transfection of GFP cDNA ( 0.25 µg per well of 24-well plate) and GFP targeted siRNA (final 10 nM, right panel) with LipoJet™ reagent leads to exceptional gene silencing on HEK293 (upper panel), Hela (middle panel) and SaoS-2 (lower panel) cells respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.

LipoJet_EG5_HEK293
LipoJet_EG5_HELA
Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with LipoJet
™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.

Data Sheet & Protocol:
- A Protocol for DNA and siRNA Transfection

- A Protocol for DNA/siRNA Co-transfection 

- A Short Protocol for DNA Transfection 

- A Short Protocol for siRNA Transfection 

Great News! We are offering "Wireless PowerPoint Presenter" to customers who purchase 5x1.0 ml LipoJet™ reagent by September 2014. Take advantage of it and don't let this chance go...

Free Powerpoint presenter

FG-3: Wireless PowerPoint Presenter with Case for purchase of 5 X 1.0 ml or more. This presenter is offered for limited time only!


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