LipoJet™ In Vitro Transfection Kit (Ver. II)

Price: $288.00  $246.00
Save: 15% off

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  • Catalog #: SL100468
  • Unit Size: 1.0 ml

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Description:
Based on our innovative and proprietary lipid-conjugation technology, LipoJet™ Transfection Kit, formulated from novel fluorinated cationic lipids, exhibits significant difference from other lipids transfection reagents in the market. LipoJet™ Transfection Kit is the most powerful yet very gentle gene delivery tool for a variety of applications including plasmid DNA and/or siRNA for most of mammalian cell types.  Compared with leading products in the market, LipoJet™ is more cost-effective and always provides higher transfection efficiency with less cytotoxicity.

Kit content:
- LipoJet™ Reagent, 1.0 ml sufficient for 1000 DNA transfections and 1000 siRNA transfections (24-well plate).

- LipoJet™ Transfection Buffer (5x ), 8.0 ml to make 40 ml of working solution (1x ).

Features: 
- Versatile: DNA transfection, siRNA transfection and DNA/siRNA co-transfection.
- Powerful: 
Outstanding efficiency on adherent cells.
- Extremely low toxicity: Improve Cell viability after transfection
- Economical: More transfections wiht less reagent
- Easy to use: One tube reaction and compitable with serum/antibiotics.
- Suitable for high-throughput application


Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Broad Transfection Spectrum for Mammalian Cell Types

Cell Line

DNA Transfection

siRNA Transfection

293, 293T
3T3, NIH 3T3
3T3-L1
A549
B16-F10
BNLCL.2
C2C12
C3H/10T1/2
Caco-2
Rat primary cardiomyocytes
CHO
COS1
COS7
Human bladder carcinomal cell
mES
iMEF
HBEC
HCT116
Hela
HepG2
HT-29
HuH-7
LNCaP
MC3T3-E1
MCF10A
MCF7
MDA-MB-231
MDCK
MEF
Human primary melanocytes
Human primary melanoma
Human immortalized microglial
mIMCD-3
MRC-5
N2A
PC3
RAW 264.7
RPE
SH-SY-5Y
SK-OV-3
U-2OS
VERO

85%
65%
30%
80%
75%
55%
80%
60%
20%
10%
80%
70%
75%
80%
50%
55%
45%
75%
80%
60%
45%
45%
45%
50%
40%
50%
50%
20%
50%
50%
50%
50%
50%
50%
68%
70%
45%
30%
75%
70%
80%
40%

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90% at 20 nM
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90% at 10 nM
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90% at 10 nM
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80 % at 20 nM
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85% at 30 nM
85% at 30 nM
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90% at 25 nM
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Examples Showing Excellent DNA and siRNA Transfection Efficiency of LipoJet™ In Vitro Transfection Kit

A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on HEK293T cell. pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into 293T cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.


A comparison of LipoJet™ reagent vs. Lipofectamine 2000 (L2K) and PolyJet™ transfection reagent on Hela cell.  pEGFP-N3 cDNA (0.125 µg/well, 0.25 µg/well and 0.5 µg/per well of 24-well plate) was transfected into Hela cells using the standard transfection protocols in presence of serum (10% FBS) with LipoJet™ (upper panel at LipoJet™/DNA (µl/µg) ratio=2), L2K (middle panel at L2K/DNA (µl/µg) ratio=3) and PolyJet™ (lower panel at at PolyJet/DNA (µl/µg) ratio=3) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.




Exceptional gene silencing of LipoJet™ reagent during DNA/siRNA co-transfection on mammalian cells. Transfection of GFP cDNA (left panel, 0.25 µg per well of 24-well plate) and co-transfection of GFP cDNA ( 0.25 µg per well of 24-well plate) and GFP targeted siRNA (final 10 nM, right panel) with LipoJet™ reagent leads to exceptional gene silencing on HEK293 (upper panel), Hela (middle panel) and SaoS-2 (lower panel) cells respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.



Excellent silencing of endogenously expressed KIF11 (also known as EG5) in HEK293 (upper panel) and Hela (lower panel) cells with LipoJet
™ reagent at 10 nM EG5 siRNA. KIF11 (also known as EG5) encodes a motor protein that belongs to the kinesin-like protein family involved in chromosome positioning and bipolar spindle formation during cell mitosis. A reduction in KIF11 levels causes mitotic arrest. LipoJet™ reagent effectively delivers EG5 siRNA (final 10 nM) to HEK293 and Hela cells, leading to more than 80% of "round-up" phenotype of HEK293 and Hela cells 24h post transfection over negative control (final 10 nM with sham EG5 siRNA). The phenotype of "rounded-up" HEK293 and Hela cells were visualized 24h post transfection with a Nikon microscope.


Transfection-related cytotoxicity of LipoJet™ DNA In Vitro Transfection Reagent vs. Lipofectamine 2000 (L2K) and PolyJet™  on Hela cell. Hela cell was transfected with the indicated transfection reagents above per the standard transfection protocols in presence of serum (10% FBS).  Total 1.0 µg of GFP DNA (pEGFP-C1)  per well of 24-well plate was transfected to Hela cell by LipoJet, L2K and PolyJet at reagent/DNA (µl/µg) ratior at 2:1, 3:1 and 3:1 respectivelyThe cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 48 hours post transfection.

Data Sheet & Protocol:
- A Protocol for DNA and siRNA Transfection

- A Prottocol for DNA/siRNA Co-transfection 


Great News! We are offering "Wireless PowerPoint Presenter" to customers who purchase 5x1.0 ml LipoJet™ reagent by September 2014. Take advantage of it and don't let this chance go...

FG-3: Wireless PowerPoint Presenter with Case for purchase of 5 X 1.0 ml or more. This presenter is offered for limited time only!


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