GenJet™ In Vitro DNA Transfection Reagent for U2OS Cells

Price: $169.00

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  • Catalog #: SL100489-OS
  • Unit Size: 1.0 ml

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Description

GenJet™ DNA In Vitro Transfection Reagent for U2OS cells is pre-optimized for transfecting U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2 OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).

Refer to the following optimal transfection conditions for maximal transfection efficiency on U2 OS cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


60~70%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
No
48 hours


EGFP
pEGFP-N3 (CMV promoter)

95%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A Picture Showing Transfection Efficiency of GenJet™ Reagent on U2 OS Cells


GenJet™ reagent is optimized for U2OS. U2OS cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection

Data Sheet




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