AAV Serotype Capsid Mutation(s) AAV2 Y730F AAV2 Y444F AAV2 Y444F+Y730F AAV2 Y444F+Y730F+Y500F AAV2 Y444F+Y730F+Y500F+Y272F AAV8 Y733F AAV8 Y447F AAV9 Y446F AAV9 Y731F AAV9 Y446F + Y731F
AAV 2 vector: Point mutations in tyrosines lead to high-efficiency transduction.
AAV2-mediated transduction of hepatocytes from normal C57BL/6 mice injected via tail vein with tyrosine-mutant capsid scAAV2-EGFP vectors. (A) Transgene expression was detected by fluorescence microscopy 2 weeks after injection of […]
Suppression of microRNA via Tough Decoy rAAV
http://signagen.com/Application_Notes/Application_Note_AAV_2015_9.pdf
The WPRE reduces readthrough transcription from retroviral vectors
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed […]
What is WPRE?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]
Search a promoter for a specific gene
http://www.genecopoeia.com/product/search/index.php?prt=22
Eukaryotic Promoter Database
EPDnew (Eukaryotic Promoter Database) – is a new collection of experimentally validated promoters in human, mouse, D. melanogaster and zebrafish genomes. Evidence comes from TSS-mapping from high-throughput expreriments such as […]
Common promoters for eukaryotes and prokaryotes
Eukaryotic Promoters Promoter Primarily used for RNA transcript Description Expression Additional considerations CMV General expression mRNA Stong mammalian expression promoter from the human cytomegalovirus Constitutive May contain an enhancer region. […]
Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG […]
Comparison of EF-1 alpha and CMV promoterd for engineering stable tumor cell lines using rAAV
Stable HT-29 clones with > 97% of all cells homogeneously expressing GFP were generated with the EF-1 alpha promoter. In contrast in clones carrying the CMV promoter, only up to […]