EPDnew (Eukaryotic Promoter Database) – is a new collection of experimentally validated promoters in human, mouse, D. melanogaster and zebrafish genomes. Evidence comes from TSS-mapping from high-throughput expreriments such as […]
Common promoters for eukaryotes and prokaryotes
Eukaryotic Promoters Promoter Primarily used for RNA transcript Description Expression Additional considerations CMV General expression mRNA Stong mammalian expression promoter from the human cytomegalovirus Constitutive May contain an enhancer region. […]
Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG […]
Comparison of EF-1 alpha and CMV promoterd for engineering stable tumor cell lines using rAAV
Stable HT-29 clones with > 97% of all cells homogeneously expressing GFP were generated with the EF-1 alpha promoter. In contrast in clones carrying the CMV promoter, only up to […]
Why EF1alpha promoter is so special?
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. […]
Where to buy DMTMM
http://www.tcichemicals.com/eshop/en/us/commodity/D2919/
DMTMM A Novel Coupling Reagent
Propylphosphonic anhydride (T3P®) is a very efficient, nontoxic coupling reagent especially suited for connecting sterically hindered amino acids. A further advantage is the easy removal of byproducts by extraction of the […]
Applications of Channelrhodopsin
Channelrhodopsins can be readily expressed in excitable cells such as neurons using a variety of transfection techniques (viral transfection, electroporation, gene gun). The light-absorbing pigment retinal is present in most […]
Discovery of Channelrhodopsin
Motility and photoorientation of microalgae have been studied over more than hundred years in many laboratories worldwide. In 1980, Ken Foster developed the first consistent theory about the functionality of […]
shRNA Database from Broad Institute
http://www.broadinstitute.org/rnai/public/gene/search