Adeno-associated Virus (AAV) - 2. page
IRES or 2A in my polycistronic expression cassette, which one is better?
When designing a gene expression vector to co-express multiple ORFs under the control of a single promoter, you can choose to place multiple ORFs behind the promoter, separated by linkers […]
Starting Materials to Run a ddPCR for rAAV Titration with An AutoDG Droplet Generator and QX 200 Droplet Reader
Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes no dUTP, Bio-Rad,1863023 10% Pluronic F-68, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, […]
Tips and tricks for performing ddPCR
Clean everything You must have a clean working area to titer your AAV by ddPCR. Droplet digital PCR is a sensitive assay, so cross contamination into a no template control […]