The efficiency of any PCR can be evaluated by performing a dilution series experiment using the target assay. We recommend a 10-fold dilution series. After properly setting the baseline and […]
Miscellaneous - 7. page
Validated Mouse EZH2 Target Region Sequence
gcgtataaagacaccacctaa
What Is ROX And Why Use It In qPCR?
To ROX or not to ROX? That is the question. I frequently see people confused about what ROX is and why it is used in qPCR. For very sensitive PCR […]
Top 10 Tips for Consistent qPCR
qPCR requires a certain amount of technical finesse to ensure consistent data across experiments. The main challenges encountered when starting out with this technique are contamination issues or inconsistency between […]
A Validated Target Region Against Mouse Tyrosine Hydroxylase (Th)
AACGGTACTGTGGCTACCGAG
A Validated Target Region Against Mouse TAZ
cagccgaatctcgcaatgaat
Validated Target Region Targeting LacZ
CTACACAAATCAGCGATTT
Validated Mouse LPCAT3 Target Region
CAGGTCAGCAGTCTAATTCG
Validated mJUNB Target Region Sequence
AAGAGTCGTCGTGATAGAAAGG
Validated siRNA against RBJP and Hes1
RBJP: CGGCAUUACUGGAUGCAGATT Hes1: CACUGAUUUUGGAUGCACUTT