Miscellaneous

Splice donor and acceptor sites are specific nucleotide sequences that define the boundaries between exons and introns during RNA splicing. Donor sites, located at the 5′ end of an intron (exon-intron boundary), […]

In a neutral pH range, pH 5 to 9, DNA molecules are quite stable. However, if the pH becomes too acidic or alkaline, DNA molecules are prone to destabilization. At […]

The pH of double-distilled water (ddH₂O) is theoretically around 7.0 — perfectly neutral — because pure water at 25°C autoionizes slightly into H₃O⁺ and OH⁻ ions. But in practice, ddH₂O […]

Here are some strategies to increase 2A linker cleavage efficiency for monoclonal antibody production:

To avoid cross-contamination in qPCR, key practices include: separating pre- and post-amplification areas with dedicated equipment, using a unidirectional workflow, including a “no template control” in each run, properly cleaning work […]

Use a unidirectional workflow during experiment setup. Separating pre- and post-amplification areas is key to preventing contamination. Prepare your PCR master mix in a template-free room (see next bullet) using reagents that never […]

The OmpA signal peptide is a 21-amino acid sequence that targets fused proteins to the Sec secretion pathway in E. coli. It can be used to increase protein yield and simplify purification […]

High-cell-density cultivation (HCDC) is a process that improves the formation of microbial biomass and products. It can be used for microorganisms such as bacteria, archaea, and yeasts. HCDC can make the fermentation process […]

To protect RNA from degradation, you can:  RNA is prone to enzymatic degradation and hydrolysis. Even at low temperatures, RNA can still be reactive, and some ribozymes can be enhanced by […]