Miscellaneous

In a neutral pH range, pH 5 to 9, DNA molecules are quite stable. However, if the pH becomes too acidic or alkaline, DNA molecules are prone to destabilization. At […]

The pH of double-distilled water (ddH₂O) is theoretically around 7.0 — perfectly neutral — because pure water at 25°C autoionizes slightly into H₃O⁺ and OH⁻ ions. But in practice, ddH₂O […]

Here are some strategies to increase 2A linker cleavage efficiency for monoclonal antibody production:

To avoid cross-contamination in qPCR, key practices include: separating pre- and post-amplification areas with dedicated equipment, using a unidirectional workflow, including a “no template control” in each run, properly cleaning work […]

Use a unidirectional workflow during experiment setup. Separating pre- and post-amplification areas is key to preventing contamination. Prepare your PCR master mix in a template-free room (see next bullet) using reagents that never […]

The OmpA signal peptide is a 21-amino acid sequence that targets fused proteins to the Sec secretion pathway in E. coli. It can be used to increase protein yield and simplify purification […]

High-cell-density cultivation (HCDC) is a process that improves the formation of microbial biomass and products. It can be used for microorganisms such as bacteria, archaea, and yeasts. HCDC can make the fermentation process […]

To protect RNA from degradation, you can:  RNA is prone to enzymatic degradation and hydrolysis. Even at low temperatures, RNA can still be reactive, and some ribozymes can be enhanced by […]

Unlike DNA, RNA is usually single-stranded. Additionally, RNA contains ribose sugars rather than deoxyribose sugars, which makes RNA more unstable and more prone to degradation. RNA is generally stable at –80° […]