Miscellaneous

1. Typical linear ranges 2. How to determine linear range experimentally 3. Practical notes

TetR (Tetracycline Repressor) rTetR (reverse TetR) Summary Table Feature TetR rTetR (reverse TetR) Binds tetO (no doxycycline) Yes No Binds tetO (with doxycycline) No Yes Gene expression (no doxycycline) OFF […]

To extract protein from inclusion bodies, you must first lyse cells to release them and then solubilize the inclusion bodies using strong denaturing agents like guanidine HCl or urea to break apart the aggregated protein. […]

Since imidazole absorbs UV radiation at 280 nm, an elution profile measured at 280 nm while purifying a 6xHis tagged protein by FPLC will show an increase in absorbance above […]

Splice donor and acceptor sites are specific nucleotide sequences that define the boundaries between exons and introns during RNA splicing. Donor sites, located at the 5′ end of an intron (exon-intron boundary), […]

In a neutral pH range, pH 5 to 9, DNA molecules are quite stable. However, if the pH becomes too acidic or alkaline, DNA molecules are prone to destabilization. At […]

The pH of double-distilled water (ddH₂O) is theoretically around 7.0 — perfectly neutral — because pure water at 25°C autoionizes slightly into H₃O⁺ and OH⁻ ions. But in practice, ddH₂O […]

Here are some strategies to increase 2A linker cleavage efficiency for monoclonal antibody production:

To avoid cross-contamination in qPCR, key practices include: separating pre- and post-amplification areas with dedicated equipment, using a unidirectional workflow, including a “no template control” in each run, properly cleaning work […]

Use a unidirectional workflow during experiment setup. Separating pre- and post-amplification areas is key to preventing contamination. Prepare your PCR master mix in a template-free room (see next bullet) using reagents that never […]