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Target sequences for generation of ATG5 or ATG7 siRNAs siRNA construct Target sequences (5′–3′) atg5 Construct 1 CAACGTGCTTTACTCTCTA Construct 2 TGAGATAACTGAACGAGAA Construct 3 ATCTGAGCTATCCAGACAA Scrambled GTCCCGGATACCTAATAAA atg7 Construct 1 ACTACAATGGTGACTCTGC Construct 2 […]

Stealth Select siRNAs targeting ​ ATG5 (no. 1; 5′-G​G​U​U​U​G​G​A​C​G​A​A​U​U​C​C​A​A​C​U​U​G​U​U​U​-3′, no. 2; 5′-G​A​U​C​A​C​A​A​G​C​A​A​C​U​C​U​G​G​A​U​G​G​G​A​U​-3′ and no. 3; 5′-G​C​C​A​U​C​A​A​U​C​G​G​A​A​A​C​U​C​A​U​G​G​A​A​U​-3′); BECN1 (no. 1; 5′-C​C​A​C​U​C​U​G​U​G​A​G​G​A​A​U​G​C​A​C​A​G​A​U​A​-3′, no. 2; 5′-G​G​A​U​G​A​U​G​A​G​C​U​G​A​A​G​A​G​U​G​U​U​G​A​A​-3′ and no. 3; 5′-A​C​A​G​U​G​A​A​U​U​U​A​A​A​C​G​A​C​A​G​C​A​G​C​U​-3′); ​ ATG7 (no. […]

Several validated siRNAs Name Sequence (5′-3′) Target SRSF1 siRNA #1 CGGGUUAAAGUUGAUGGGCCCAGAA exon 4 SRSF1 siRNA #2 UAGCAGAUCUCGCUCUCGUACAUAA exon 4 SRSF2 siRNA #1 UCGCCUUCGUUCGCUUUCACGACAA exon 1 SRSF2 siRNA #2 CCCAGUGUCCAAGAGGGAAUCCAAA exon […]

Full Sequence of hAsCpf1-NLS-3xHA Sequence: ATGACACAGTTCGAGGGCTTTACCAACCTGTATCAGGTGAGCAAGACACTGCGGTTTGAGCTGATCCCACAGGGCAAGACCCTGAAGCACATCCAGGAGCAGGGCT TCATCGAGGAGGACAAGGCCCGCAATGATCACTACAAGGAGCTGAAGCCCATCATCGATCGGATCTACAAGACCTATGCCGACCAGTGCCTGCAGCTGGTGCAGCT GGATTGGGAGAACCTGAGCGCCGCCATCGACTCCTATAGAAAGGAGAAAACCGAGGAGACAAGGAACGCCCTGATCGAGGAGCAGGCCACATATCGCAATGCCA TCCACGACTACTTCATCGGCCGGACAGACAACCTGACCGATGCCATCAATAAGAGACACGCCGAGATCTACAAGGGCCTGTTCAAGGCCGAGCTGTTTAATGGCAA GGTGCTGAAGCAGCTGGGCACCGTGACCACAACCGAGCACGAGAACGCCCTGCTGCGGAGCTTCGACAAGTTTACAACCTACTTCTCCGGCTTTTATGAGAACAGG AAGAACGTGTTCAGCGCCGAGGATATCAGCACAGCCATCCCACACCGCATCGTGCAGGACAACTTCCCCAAGTTTAAGGAGAATTGTCACATCTTCACACGCCTGA TCACCGCCGTGCCCAGCCTGCGGGAGCACTTTGAGAACGTGAAGAAGGCCATCGGCATCTTCGTGAGCACCTCCATCGAGGAGGTGTTTTCCTTCCCTTTTTATAAC CAGCTGCTGACACAGACCCAGATCGACCTGTATAACCAGCTGCTGGGAGGAATCTCTCGGGAGGCAGGCACCGAGAAGATCAAGGGCCTGAACGAGGTGCTGAAT CTGGCCATCCAGAAGAATGATGAGACAGCCCACATCATCGCCTCCCTGCCACACAGATTCATCCCCCTGTTTAAGCAGATCCTGTCCGATAGGAACACCCTGTCTTT CATCCTGGAGGAGTTTAAGAGCGACGAGGAAGTGATCCAGTCCTTCTGCAAGTACAAGACACTGCTGAGAAACGAGAACGTGCTGGAGACAGCCGAGGCCCTGTT TAACGAGCTGAACAGCATCGACCTGACACACATCTTCATCAGCCACAAGAAGCTGGAGACAATCAGCAGCGCCCTGTGCGACCACTGGGATACACTGAGGAATGC CCTGTATGAGCGGAGAATCTCCGAGCTGACAGGCAAGATCACCAAGTCTGCCAAGGAGAAGGTGCAGCGCAGCCTGAAGCACGAGGATATCAACCTGCAGGAGA TCATCTCTGCCGCAGGCAAGGAGCTGAGCGAGGCCTTCAAGCAGAAAACCAGCGAGATCCTGTCCCACGCACACGCCGCCCTGGATCAGCCACTGCCTACAACCC TGAAGAAGCAGGAGGAGAAGGAGATCCTGAAGTCTCAGCTGGACAGCCTGCTGGGCCTGTACCACCTGCTGGACTGGTTTGCCGTGGATGAGTCCAACGAGGTG GACCCCGAGTTCTCTGCCCGGCTGACCGGCATCAAGCTGGAGATGGAGCCTTCTCTGAGCTTCTACAACAAGGCCAGAAATTATGCCACCAAGAAGCCCTACTCCG TGGAGAAGTTCAAGCTGAACTTTCAGATGCCTACACTGGCCTCTGGCTGGGACGTGAATAAGGAGAAGAACAATGGCGCCATCCTGTTTGTGAAGAACGGCCTGT ACTATCTGGGCATCATGCCAAAGCAGAAGGGCAGGTATAAGGCCCTGAGCTTCGAGCCCACAGAGAAAACCAGCGAGGGCTTTGATAAGATGTACTATGACTACT TCCCTGATGCCGCCAAGATGATCCCAAAGTGCAGCACCCAGCTGAAGGCCGTGACAGCCCACTTTCAGACCCACACAACCCCCATCCTGCTGTCCAACAATTTCATC GAGCCTCTGGAGATCACAAAGGAGATCTACGACCTGAACAATCCTGAGAAGGAGCCAAAGAAGTTTCAGACAGCCTACGCCAAGAAAACCGGCGACCAGAAGGGC TACAGAGAGGCCCTGTGCAAGTGGATCGACTTCACAAGGGATTTTCTGTCCAAGTATACCAAGACAACCTCTATCGATCTGTCTAGCCTGCGGCCATCCTCTCAGTA TAAGGACCTGGGCGAGTACTATGCCGAGCTGAATCCCCTGCTGTACCACATCAGCTTCCAGAGAATCGCCGAGAAGGAGATCATGGATGCCGTGGAGACAGGCAA GCTGTACCTGTTCCAGATCTATAACAAGGACTTTGCCAAGGGCCACCACGGCAAGCCTAATCTGCACACACTGTATTGGACCGGCCTGTTTTCTCCAGAGAACCTGG CCAAGACAAGCATCAAGCTGAATGGCCAGGCCGAGCTGTTCTACCGCCCTAAGTCCAGGATGAAGAGGATGGCACACCGGCTGGGAGAGAAGATGCTGAACAAGA AGCTGAAGGATCAGAAAACCCCAATCCCCGACACCCTGTACCAGGAGCTGTACGACTATGTGAATCACAGACTGTCCCACGACCTGTCTGATGAGGCCAGGGCCCT GCTGCCCAACGTGATCACCAAGGAGGTGTCTCACGAGATCATCAAGGATAGGCGCTTTACCAGCGACAAGTTCTTTTTCCACGTGCCTATCACACTGAACTATCAGG […]

PLEKHM1 (cDNA) # 1 GCAAAGUCCUGGCAUCCUA # 2 GAACGCGUCUCUGUACGAG VPS11 (cDNA) GAAACGAGAUCUCCAUGAU VPS41 (cDNA) GAGAAUGAAUGUAGAGAU RAB7A (cDNA) # 1 GUACAAAGCCACAAUAGGA # 2 AAACGGAGGUGGAGCUGUA

GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGAATCCAGCGATCCGAGTTCAAATCTCGGTGGAACCT

Mouse Rab9-siRNA sense, CGGCGACUAUCCUUACUUUTT and antisense, AAAGUAAGGAUAGUCGCCGTT ; mouse Beclin 1-siRNA sense, GAGUUGCCGUUAUACUGUUCU and antisense, AACAGUAUAACGGCAACUCCU ; mouse LAMP-2A-siRNA sense, GAGGAGUACUUAUUCUAGUGU and antisense, ACUAGAAUAAGUACUCCUCCC ; mouse Hsc70-siRNA sense, CGAUGAAGCUGUUGCCUAUTT and antisense, AUAGGCAACAGCUUCAUCGGG; […]