1 atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc 61 gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac 121 aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac 181 aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac 241 cagcagctca […]
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AAV VP1 Sequence Blast Serotype PHP.B vs Serotype PHP.eB
Query 1 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLD 60 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLD Sbjct 1 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLD 60 Query 61 KGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ 120 KGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ Sbjct 61 KGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQ 120 Query 121 AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTE 180 AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTE Sbjct 121 AKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTE 180 Query 181 SVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVI […]
Synthetic AAV-PHP.eB Capsid Protein Gene, Complete Cds
1 atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc 61 gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac 121 aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac 181 aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac 241 cagcagctca […]
NCOR1 and MSTN Validated Target Region Sequence
NCOR1 target: GCTCTCAAAGTTCAGACTCTT MSTN target: GATTGGATTATCGCTCCTAAA
gpNa V 1.7-targeting shRNA
5′-GGGCATAGATTACGTGAAA TTCAAGAGA TTTCACGTAATCTATGCCC-3′
Cas9 Species/Variants and PAM Sequences
Species/Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 NGG SpCas9 D1135E variant NGG (reduced NAG binding) SpCas9 VRER variant NGCG SpCas9 EQR variant NGAG SpCas9 VQR variant NGAN or […]