CRISPR-EZ Video Protocol
All posts by signagen - 18. page
Multiple sgRNAs with overlapping sequences enhance CRISPR/Cas9-mediated knock-in efficiency
Abstract The CRISPR/Cas9 system is widely applied in genome engineering due to its simplicity and versatility. Although this has revolutionized genome-editing technology, knockin animal generation via homology directed repair (HDR) […]
Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells
Abstract The targeting efficiency of knockin sequences via homologous recombination (HR) is generally low. Here we describe a method we call Tild-CRISPR (targeted integration with linearized dsDNA-CRISPR), a targeting strategy […]
CRISPR Genome Editing in Mice from He Lab at UC Berkeley
CRISPR-mediated mouse genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and sgRNA into zygotes to achieve editing in one step. We developed a simple and economic electroporation-based strategy, […]
Validated GPAM Target Region Sequence
Validated GPAM Target Region Sequence: GACGGAGGCTCGATGAAAC GACTGTGGGTTCAAACCTA
Validated Tbk1 Target Region Sequence
Validated Tbk1 Target Region Sequence: GCAGAGCACTTCCAACCATCT
Several Validated siRNA
siRNA Species Target sequence PPARα mouse 5´-GGGUCAUACUCGCGGGAAAdtdt-3´ PPARα mouse 5´-GGCUGAAGCUGGUGUACGAdtdt-3´ PPARα mouse 5´-GGGCAAGAGAAUCCACGAAdtdt-3´ PPARα mouse 5´-ACACAAUGCAAUUCGCUUUdtdt-3´ GYK […]
Argininosuccinate Lyase (ALS) Validated Target Region
CACCTTCAAACTGAACTCCAA AGGAGGCTGCTGTGTGTTT
Validated gpNaV1.7 shRNA
GGGCATAGATTACGTGAAATTCAAGAGATTTCACGTAATCTATGCCC
Validated Target Region for Mouse STX18
ACGACTAACTGGTGAATG