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All posts by signagen - 13. page

Commonly Used Primers

3’AOX1 GCAAATGGCATTCTGACATCC For Pichia vectors with AOX1 terminator, reverse primer 5’AOX1 GACTGGTTCCAATTGACAAGC For Pichia vectors with AOX1 promoter, forward primer 35S promoter CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer AC5 ACACAAAGCCGCTCCATCAG […]

Sim1 Specific Promoter for PVN Neurons

1 GGTACCGAGG CGCGCAGCCG TGGGGAAATC CTGGCCACTG TCTGGGATGC 51 TATTCGCACG CGTTCCTGGA GCCTCGGCTA GCCAGCCCCC GCAAGGCCGC 101 ACGCTGTGCG CAGACACCCG AGTCTAGGGA GCGCGAGTGT CACTCGGCAC 151 GCCGAAGACC TCGGGGTTTG GAGCGGCGGC GGCAGCGGGG CAGCGGCGAC 201 CCCGCGCCCG GGGCTCATTA GGAGCGGAGC TGCCAGGCTC ACTGTAGGAC […]

Wt AAV P5 Promoter for Rep

ggaggggtggagtcgtgacgtgaattacgtcatagggttagggaggtcctgtattagaggtcacgtgagtgttttgcgacattttgcgacaccatgtggtcacgctgggtatttaagcccg agtgagcacgcagggtctccattttgaagcgggaggtttgaac

rcAAV Assay via PCR

PCR and sequencing. The following primers were used for PCR amplification for analysis of rcAAV: D region, 5′-CTCCATCACTAGGGGTTCC-3′ (AAV2 nt 126 to 144); rep, 5′-GGCAGATGCCCGTCAAGGT-3′ (AAV2 nt 379 to 361); and cap, 5′-CAGAGATGTGTACCTTCAG-3′ […]