Daily Archives: January 1, 2024

For a 20µl reaction: Component   Volume (µl)   Final Concentration H2O 12.9   10x homemade core buffer 2 1x 2mM dNTP mix 2 0.2mM 20µM primer mix 0.5 500nM […]

Reagent Volume (for 150 µL) qPCR mix (2×) 100 µL Sense primer (1 µM) 2 µL Antisense primer (1 µM) 2 µL Fluorescein (1 µM) 2 µL SYBR Green (40×) […]