Monthly Archives: March 2016

The termination sequence for the U6 promoter is a series of T’s (5-6) in a row.

The “G” that you mentioned is in fact the “preferred” transcription start site, however, this can be replaced by any other nucleotide. It’s just that U6 prefers “G” as the […]

Background Decoy and Tough Decoy (TuD) RNAs are novel technologies for regulating RNA interference (Haraguchi et al., Nucleic Acids Res. 2009; Xie et al., Nature 2012). In short, they are […]

5′-GATCCCCGAAAGTACATTTTCAACTGTTCAAGAGACAGTTGAAAATGTACTTTCTTTTTGGAAA-3′ (HIPK2-1376 sense), 5′-AGCTTTTCCAAAAAGAAAAGTACATTTTCAACGTCTCTTGAACAGTTGAAAATGTACTTTCGGG-3′ (HIPK2-1376 antisense), 5′-GATCCCCGAACCACACGTGCTTGGTCTTCAAGAGAGACCAAGCACGTGTGGTTCTTTTTGGAAA-3′ (HIpk2-789 sense), and 5′-AGCTTTTCCAAAAAGAACCACACGTGCTTGGTCTCTCTTGAAGACCAAGCACGTGTGGTTCGGG-3′ (HIPK2-789 antisense). 5′-GATCCCCCAACAGGAGAGTCATTGTTTTCAAGAGAAACAATGACTCTCCTGTTGTTTTTGGAAA-3′ (Gal3-551, sense), 5′-AGCTTTTCCAAAAACAACAGGAGAGTCATTGTTTCTCTTGAAAACAATGACTCTCCTGTTGGGG-3′ (Gal3-551, antisense), 5′-GATCCCCACCTTACATGTGTAAAGGTTTCAAGAGAACCTTTACACATGTAAGGTTTTTTGGAAA-3′ (Gal3-845, sense), and 5′-AGCTTTTCCAAAAAACCTTACATGTGTAAAGGTTCTCTTGAAACCTTTACACATGTAAGGTGGG-3′ (Gal3-845, antisense).

Target sequences for generation of ATG5 or ATG7 siRNAs siRNA construct Target sequences (5′–3′) atg5 Construct 1 CAACGTGCTTTACTCTCTA Construct 2 TGAGATAACTGAACGAGAA Construct 3 ATCTGAGCTATCCAGACAA Scrambled GTCCCGGATACCTAATAAA atg7 Construct 1 ACTACAATGGTGACTCTGC Construct 2 […]

Stealth Select siRNAs targeting ​ ATG5 (no. 1; 5′-G​G​U​U​U​G​G​A​C​G​A​A​U​U​C​C​A​A​C​U​U​G​U​U​U​-3′, no. 2; 5′-G​A​U​C​A​C​A​A​G​C​A​A​C​U​C​U​G​G​A​U​G​G​G​A​U​-3′ and no. 3; 5′-G​C​C​A​U​C​A​A​U​C​G​G​A​A​A​C​U​C​A​U​G​G​A​A​U​-3′); BECN1 (no. 1; 5′-C​C​A​C​U​C​U​G​U​G​A​G​G​A​A​U​G​C​A​C​A​G​A​U​A​-3′, no. 2; 5′-G​G​A​U​G​A​U​G​A​G​C​U​G​A​A​G​A​G​U​G​U​U​G​A​A​-3′ and no. 3; 5′-A​C​A​G​U​G​A​A​U​U​U​A​A​A​C​G​A​C​A​G​C​A​G​C​U​-3′); ​ ATG7 (no. […]

Several validated siRNAs Name Sequence (5′-3′) Target SRSF1 siRNA #1 CGGGUUAAAGUUGAUGGGCCCAGAA exon 4 SRSF1 siRNA #2 UAGCAGAUCUCGCUCUCGUACAUAA exon 4 SRSF2 siRNA #1 UCGCCUUCGUUCGCUUUCACGACAA exon 1 SRSF2 siRNA #2 CCCAGUGUCCAAGAGGGAAUCCAAA exon […]