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Month February 2016
hAsCpf1-NLS-3xHA Sequence
Full Sequence of hAsCpf1-NLS-3xHA Sequence: ATGACACAGTTCGAGGGCTTTACCAACCTGTATCAGGTGAGCAAGACACTGCGGTTTGAGCTGATCCCACAGGGCAAGACCCTGAAGCACATCCAGGAGCAGGGCT TCATCGAGGAGGACAAGGCCCGCAATGATCACTACAAGGAGCTGAAGCCCATCATCGATCGGATCTACAAGACCTATGCCGACCAGTGCCTGCAGCTGGTGCAGCT GGATTGGGAGAACCTGAGCGCCGCCATCGACTCCTATAGAAAGGAGAAAACCGAGGAGACAAGGAACGCCCTGATCGAGGAGCAGGCCACATATCGCAATGCCA TCCACGACTACTTCATCGGCCGGACAGACAACCTGACCGATGCCATCAATAAGAGACACGCCGAGATCTACAAGGGCCTGTTCAAGGCCGAGCTGTTTAATGGCAA GGTGCTGAAGCAGCTGGGCACCGTGACCACAACCGAGCACGAGAACGCCCTGCTGCGGAGCTTCGACAAGTTTACAACCTACTTCTCCGGCTTTTATGAGAACAGG AAGAACGTGTTCAGCGCCGAGGATATCAGCACAGCCATCCCACACCGCATCGTGCAGGACAACTTCCCCAAGTTTAAGGAGAATTGTCACATCTTCACACGCCTGA TCACCGCCGTGCCCAGCCTGCGGGAGCACTTTGAGAACGTGAAGAAGGCCATCGGCATCTTCGTGAGCACCTCCATCGAGGAGGTGTTTTCCTTCCCTTTTTATAAC CAGCTGCTGACACAGACCCAGATCGACCTGTATAACCAGCTGCTGGGAGGAATCTCTCGGGAGGCAGGCACCGAGAAGATCAAGGGCCTGAACGAGGTGCTGAAT CTGGCCATCCAGAAGAATGATGAGACAGCCCACATCATCGCCTCCCTGCCACACAGATTCATCCCCCTGTTTAAGCAGATCCTGTCCGATAGGAACACCCTGTCTTT…
Several Validated siRNAs
PLEKHM1 (cDNA) # 1 GCAAAGUCCUGGCAUCCUA # 2 GAACGCGUCUCUGUACGAG VPS11 (cDNA) GAAACGAGAUCUCCAUGAU VPS41 (cDNA) GAGAAUGAAUGUAGAGAU…
Gln tRNA Promoter
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGAATCCAGCGATCCGAGTTCAAATCTCGGTGGAACCT
AAV-TBG-saCas9-U6-sgRNA Ready to Package
AAV-TBG-saCas9-U6-sgRNA Ready to Package
AAV-tRNA-sgRNA-CMV-hAsCpf1 Ready to Package
AAV-tRNA-sgRNA-CMV-hAsCpf1 Ready to Package
Several Validated siRNAs
Mouse Rab9-siRNA sense, CGGCGACUAUCCUUACUUUTT and antisense, AAAGUAAGGAUAGUCGCCGTT ; mouse Beclin 1-siRNA sense, GAGUUGCCGUUAUACUGUUCU and antisense,…
Full Sequence of mt-mKeima
ATGCATCTCGAGGCCACCATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGATCCAT TCGTTGCCGCCGGAGGGGAAGCTTGGGATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCC AAGATCCATTCGTTGCCGCCGGAGGGGAAGCTCGGGggatccGCCATGGTGAGCGTGATCGCCAAGCAGATGACCTACAAGGTGTACATGAGCGGCACC GTGAACGGCCACTACTTCGAGGTGGAGGGCGACGGCAAGGGCAAGCCCTACGAGGGCGAGCAGACCGTGAAGCTGACCGTGACCAAGGGTGGCCCCC TGCCCTTCGCCTGGGACATCCTGAGCCCCCAGCTCCAGTACGGCAGCATCCCCTTCACCAAGTACCCCGAGGACATCCCCGACTACTTCAAGCAGAGCTT CCCCGAGGGCTACACCTGGGAGCGCAGCATGAACTTCGAGGACGGCGCCGTGTGCACCGTGAGCAACGACAGCAGCATCCAGGGCAACTGCTTCATCT ACAACGTGAAGATCAGCGGCGAGAACTTCCCCCCCAACGGCCCCGTGATGCAGAAGAAGACCCAGGGCTGGGAGCCCAGCACCGAGCGCCTGTTCGC CCGCGACGGAATGCTGATCGGCAACGACTACATGGCCCTGAAGCTGGAGGGCGGCGGCCACTACCTGTGCGAGTTCAAGAGCACCTACAAGGCCAAGA AGCCCGTGAGGATGCCCGGCCGCCACGAGATCGACCGCAAGCTGGACGTGACCAGCCACAACCGCGACTACACCAGCGTGGAGCAGTGCGAGATCGCC ATCGCCCGCCACTCCCTGCTGGGCTAA
AAV-EF1a-FRT-mCherry-WPRE and AAV-CAG-FRT-GFP-WPRE Ready to Package
AAV-EF1a-FRT-mCherry-WPRE and AAV-CAG-FRT-GFP-WPRE Ready to Package
siRNAs targeting human Caspase
Human Caspase-1 target region: AAGGGGCACAGGCATGCCAAA Human Apaf-1 target region: : AATTGGTGCACTTTTACGTGA Human Caspase-2 target region: AAACAGCTGTTGTTGAGCGA
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