Though there are several virus delivery systems available today for gene therapy, lentivirus is considered one of the best methods. Several features make lentivirus preferable especially the ability to infect […]
Overview of Lentivirus Pseudotypes
Family Genus Species Vector References Rhabdoviridae Vesiculovirus Vesicular stomatitis virus (Indiana virus) HIV-1 [Akkina, 1996][Naldini, 1996][Reiser, 1996] HIV-2 [Poeschla, 1998a] FIV [Poeschla, 1998b] EIAV [Olsen, 1998] BIV [Berkowitz, 2001a] JDV […]
2nd vs 3rd Generation Lentiviral Production Systems
Feature 2nd Generation 3rd Generation Transfer Plasmid Can be packaged ONLY by a second generation packaging system that includes TAT Can be packaged by both 2nd and 3rd generation packaging […]
rAAV Vector Tropism
rAAV Vector Tropism: rAAV Serotype Applications (X = Recommended for use) Skeletal muscle Heart Lung CNS / Eye Liver Adipose AAV1 X X X X – – AAV2 – – […]
Real Time PCR Dyes
Dye Structure Color Vendor Cat # Price Features SYBR Green 1 Green unstable at 4 °C SYTO-82 Orange does not inhibit PCR, show no preferential binding to GC rich sequences […]
GCaMP6 calcium indicators
Name Description Addgene Plasmid ID Reference CMV-GCaMP6s improved SNR, slower kinetics 40753 Chen et al., 2013 CMV-GCaMP6m improved SNR, intermediate kinetics 40754 Chen et al., 2013 CMV-GCaMP6f improved SNR, faster […]
Cre-inducible Adeno-associated Virus: DIO-AAV
For cell type-specific targeting and to capitalize on the large number of Cre driver lines with the flexible virus injection/fiberoptic approach, we have developed a tool we call DIO-AAV (doublefloxed […]
SignaGen’s Ad.MAX™ Adenovirus Expression System for Maximum Adenovirus Production
Ad.MAX™ Technology for Maximum Adenovirus Production: Ad.MAX™ technology was developed by genetically modifying virus packaging cell, HEK293 cell and adenoviral shuttle vector or adenoviral genome for maximum adenovirus production. The […]
Primers for Lentivirus Titration using qRT-PCR
Vector and region amplified Primers and probes dedicated for the amplification of lentiviral or transgenic DNA sequences Primers and probes dedicated for the amplification of internal control DNAs (cellular genome […]
Fusion Cloning, Phusion Cloning, Pfusion Cloning and Confusion PCR Cloning
In-Fusion PCR Cloning: Clontech, http://www.freepatentsonline.com/7575860.html Cold Fusion Cloning Kit: SBI Fast Seamless Cloning Kit: Dogene CloneEZ Kit: Genescript GENEART Seamless Cloning and Assembly Kit: Invitrogen SLIC uses the 3′ […]