Instructions: Please select a siRNA database from the pull down menu. At present we offer control duplexes and pre-designed siRNAs for over 17,000 reviewed genes based on the NCBI Refseq […]
miRBase: the microRNA database
miRBase provides the following services: The miRBase database is a searchable database of published miRNA sequences and annotation. Each entry in the miRBase Sequence database represents a predicted hairpin portion […]
How to locate promoter sequence for a specific gene
Many people have problem identifying or predicting the promoter sequence of a gene, or don’t know how to get the actual sequence for analysis such as primer design, transcription factor […]
List of Protein Tags
Peptide tags AviTag, a peptide allowing biotinylation by the enzyme BirA and so the protein can be isolated by streptavidin (GLNDIFEAQKIEWHE) Calmodulin-tag, a peptide bound by the protein calmodulin (KRRWKKNFIAVSAANRFKKISSSGAL) […]
Alternatives to Fetal Bovine Serum in Cell Culture media, Challenges and Perspectives
Dr. Gerhard Gstraunthaler recently presented a seminar with the above title. Gerhard carries a faculty appointment with the Department of Physiology at Innsbruck Medical University, Austria. Gerhard is an advocate of serum-free […]
Role of Serum in Cell Culture Media
Serum provides > growth factors and hormones > binding and transport proteins > attachment and spreading factors > additional amino acids, vitamins and trace elements > fatty acids and lipids > […]
Lentivirus Production Protocol
Supplies: 293FT Cells (Invitrogen: R700-07) T-225 tissue culture flasks (Nunc: 159934) T-75 tissue culture flasks (Nunc: 156499) 500 cm2 tissue culture plates (Nunc: 166508) Ultracentrifuge tubes (Beckman Culture: 344058) DMEM […]
Plasmids for Optogenetics Sensors from Addgene
Addgene’s repository contains a variety of genetically encoded biosensors to monitor and measure fluctuations in molecular signals in cells, including those to monitor calcium, glutamate, pH, and voltage. These optogenetics […]
Optogenetics rAAVs
INTRSECT (INTronic Recombinase Sites Enabling Combinatorial Targeting)-related constructs A versatile single-AAV system for selective expression that is conditional upon multiple cell-type features (such as wiring or genetic type) related by […]