Addgene’s repository contains a variety of genetically encoded biosensors to monitor and measure fluctuations in molecular signals in cells, including those to monitor calcium, glutamate, pH, and voltage. These optogenetics […]
Optogenetics rAAVs
INTRSECT (INTronic Recombinase Sites Enabling Combinatorial Targeting)-related constructs A versatile single-AAV system for selective expression that is conditional upon multiple cell-type features (such as wiring or genetic type) related by […]
Fetal Bovine Serum – the Tipping Point
Users of US Fetal Bovine Serum have been watching for months and months how prices climb up along with a constantly growing shortage and with no end in sight. Yet […]
Fetal Bovine Serum Availability – How do Regulations Affect It?
Now we will talk about the influence of various regulations on the availability of Fetal Bovine Serum. It is a fact that import and export regulations have direct influence on […]
>400 Pre-made Ready to Use rAAVs from SignaGen
All the following pre-made AAVs were packaged in serotype 1**, 2, 5, 6, 8** and 9** and super purified via 2xCsCl ultra-centrifugation in in-vivo grade. All the pre-packaged rAAVs were […]
Summary of clinical trials using AAV gene transfer
Disease Transgene product Serotype Route of administration Clinical trial ClinicalTrials.gov identifier Refs AAV clinical trials for inherited diseases α1 antitrypsin deficiency α1 antitrypsin AAV2 Intramuscular Phase I/II NCT00377416 101,102 AAV1 […]
Key properties of a viral vector
Viral vectors are tailored to their specific applications but generally share a few key properties: Safety: Although viral vectors are occasionally created from pathogenic viruses, they are modified in such […]
Pre-made Lentivirus from GeneCopoeia
Positive and negative controls: eGFP eYFP eCFP mCherry PLUM Firefly luciferase Renilla luciferase Gaussia luciferase Firefly luciferase + eGFP/mCherry Renilla luciferase + eGFP/mCherry Firefly luciferase + IRES-mCherry Renilla luciferase + […]
mRFP expression in neurones and glia in dorsal vagal and hypoglossal motor nuclei
Representative images are shown for the experimental conditions evaluated. mRFP-expressing cells transduced with either AVV- or LVV-CMV-mRFP are shown in the left hand panels; IR for NeuN or GFAP in […]
WHAT ARE SOME DIFFERENT WAYS TO TITER LENTIVIRUS?
Functional titer is defined as the number of functional vector particles required to infect a cell, present in a volume. There are several methods for measuring titer. Some are more […]