The following siRNA sequences were efficacious in knocking down dystonin-a1 and dystonin-a2 expression, respectively: Group A: Sense: 5’-ACAUGUACGUGGAGGAGCA-3’ / Antisense: 5’-UGCUCCUCCACGUACAUGU-3’; Sense: 5’-CAAGCAUGAGAGAUCCAAA-3’ / Antisense: 5’-UUUGGAUCUCUCAUGCUUG-3’; Group B: Sense: 5’-CAAGCAUGAGAGAUCCAAA-3’ […]
Mouse dystonin shRNA validated
Validated mouse dystonin shRNA: CCGGCCCTTGTCAAACTCTACGAAACTCGAGTTTCGTAGAGTTTGACAAGGGTTTTTG
Validated siRNA Targeting Human 15-PGDH
UUGCACAGCAGCCGGUUUAUU
mPDK4 Validated siRNA
mPDK4 Validated siRNA CCGUCUCUACGCCAAGUAUTT
rAAV Serotype 3B VP1
ATGGCTGCTGACGGTTATCTTCCAGATTGGCTCGAGGACAACCTTTCTGAAGGCATTCGTGAGTGGTGGGCTCTGAAACCTGGAGT CCCTCAACCCAAAGCGAACCAACAACACCAGGACAACCGTCGGGGTCTTGTGCTTCCGGGTTACAAATACCTCGGACCCGGTAAC GGACTCGACAAAGGAGAGCCGGTCAACGAGGCGGACGCGGCAGCCCTCGAACACGACAAAGCTTACGACCAGCAGCTCAAGGC CGGTGACAACCCGTACCTCAAGTACAACCACGCCGACGCCGAGTTTCAGGAGCGTCTTCAAGAAGATACGTCTTTTGGGGGCAAC CTTGGCAGAGCAGTCTTCCAGGCCAAAAAGAGGATCCTTGAGCCTCTTGGTCTGGTTGAGGAAGCAGCTAAAACGGCTCCTGGA AAGAAGAGGCCTGTAGATCAGTCTCCTCAGGAACCGGACTCATCATCTGGTGTTGGCAAATCGGGCAAACAGCCTGCCAGAAAA AGACTAAATTTCGGTCAGACTGGCGACTCAGAGTCAGTCCCAGACCCTCAACCTCTCGGAGAACCACCAGCAGCCCCCACAAGTT TGGGATCTAATACAATGGCTTCAGGCGGTGGCGCACCAATGGCAGACAATAACGAGGGTGCCGATGGAGTGGGTAATTCCTCAGG AAATTGGCATTGCGATTCCCAATGGCTGGGCGACAGAGTCATCACCACCAGCACCAGAACCTGGGCCCTGCCCACTTACAACAACC ATCTCTACAAGCAAATCTCCAGCCAATCAGGAGCTTCAAACGACAACCACTACTTTGGCTACAGCACCCCTTGGGGGTATTTTGAC TTTAACAGATTCCACTGCCACTTCTCACCACGTGACTGGCAGCGACTCATTAACAACAACTGGGGATTCCGGCCCAAGAAACTCAG CTTCAAGCTCTTCAACATCCAAGTTAAAGAGGTCACGCAGAACGATGGCACGACGACTATTGCCAATAACCTTACCAGCACGGTTC AAGTGTTTACGGACTCGGAGTATCAGCTCCCGTACGTGCTCGGGTCGGCGCACCAAGGCTGTCTCCCGCCGTTTCCAGCGGACGTCT TCATGGTCCCTCAGTATGGATACCTCACCCTGAACAACGGAAGTCAAGCGGTGGGACGCTCATCCTTTTACTGCCTGGAGTACTTCCC TTCGCAGATGCTAAGGACTGGAAATAACTTCCAATTCAGCTATACCTTCGAGGATGTACCTTTTCACAGCAGCTACGCTCACAGCCAG AGTTTGGATCGCTTGATGAATCCTCTTATTGATCAGTATCTGTACTACCTGAACAGAACGCAAGGAACAACCTCTGGAACAACCAACC AATCACGGCTGCTTTTTAGCCAGGCTGGGCCTCAGTCTATGTCTTTGCAGGCCAGAAATTGGCTACCTGGGCCCTGCTACCGGCAAC AGAGACTTTCAAAGACTGCTAACGACAACAACAACAGTAACTTTCCTTGGACAGCGGCCAGCAAATATCATCTCAATGGCCGCGAC TCGCTGGTGAATCCAGGACCAGCTATGGCCAGTCACAAGGACGATGAAGAAAAATTTTTCCCTATGCACGGCAATCTAATATTTGGC AAAGAAGGGACAACGGCAAGTAACGCAGAATTAGATAATGTAATGATTACGGATGAAGAAGAGATTCGTACCACCAATCCTGTGGC AACAGAGCAGTATGGAACTGTGGCAAATAACTTGCAGAGCTCAAATACAGCTCCCACGACTAGAACTGTCAATGATCAGGGGGCCT TACCTGGCATGGTGTGGCAAGATCGTGACGTGTACCTTCAAGGACCTATCTGGGCAAAGATTCCTCACACGGATGGACACTTTCATCC TTCTCCTCTGATGGGAGGCTTTGGACTGAAACATCCGCCTCCTCAAATCATGATCAAAAATACTCCGGTACCGGCAAATCCTCCGACG ACTTTCAGCCCGGCCAAGTTTGCTTCATTTATCACTCAGTACTCCACTGGACAGGTCAGCGTGGAAATTGAGTGGGAGCTACAGAAAG AAAACAGCAAACGTTGGAATCCAGAGATTCAGTACACTTCCAACTACAACAAGTCTGTTAATGTGGACTTTACTGTAGACACTAATGG TGTTTATAGTGAACCTCGCCCTATTGGAACCCGGTATCTCACACGAAACTTGTAA
Three Validated siRNAs
COX-2 siRNA: 5-AACTGCTCAACACCGGAATTTTT-3 COX-1 siRNA: 5-AAGTGCCATCCAAACTCTATCTT-3 15-PGDH siRNA: 5-AACAGTGGTGTGAGACTGAAT-3
Validated siRNA Targeting 15-PGDH and HIF-alpha
15-PGDH and Scramble control siRNA sequence: 15-PGDH: (NM_001034419.2) 5′-GCUUUGAA UGGUGCUAUUATT-3′ Scramble: 5′-UUCUCCGAACGUGUCAC GUTT-3′ HIF-1α siRNA: (NM_174339.3) 5′-GCUCCUGAGGAAGAACUAATT-3′ Scramble: 5′-UUCUCCG AACGUGUCACGUTT-3′
15-PGDH-specific shRNAs Target Region Sequence
15PGDH-specific shRNAs target region sequence: 1) CACAGCCATCCTTGAATCAAT 2) GAAGGCGGCATCATTATCAAT 3) GCAACAACTGAGAGACACTTT 4) CTGGAGTGAATAATGAGAAA 5) ACTCATAACAACACAGACATA