Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Using nanopore sequencing, a single molecule of […]
The Principle of Transformation
As DNA is a highly hydrophilic molecule, it usually cannot pass through the cell membrane of bacteria. Hence, to make bacteria capable of internalizing genetic material, they must be made […]
IRES or 2A in my polycistronic expression cassette, which one is better?
When designing a gene expression vector to co-express multiple ORFs under the control of a single promoter, you can choose to place multiple ORFs behind the promoter, separated by linkers […]
How Orbital Diameter and Shaker Agitation Rate Affect Bacteria Growth
A common use for orbital shakers is to grow bacteria for a variety of purposes, including the production of proteins and genetic matter and for the study of bacteria themselves. […]