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Universal Primers for qPCR Titration of rAAV

Primers Targeting ITR: FWD primer, 5′-GGAACCCCTAGTGATGGAGTT REV primer, 5′-CGGCCTCAGTGAGCGA qPCR reaction conditions:  98 ℃ 3 min / 98 ℃ 15 sec, 60 ℃ 30 sec / Repeat 39x / Melt […]

EglN2 Validated siRNAs

EglN2-1 5′-GACTATATCGTGCCCTGCATG-3′; EglN2-4 5′-GCCACTCTTTGACCGGTTGCT-3′; EGLN1 5′-AGCTCCTTCTACTGCTGCA-3′; EGLN3 5′-CAGGTTATGTTCGCCACGT-3′

Validated siRNA Knocking Down Dystonin-a1 and Dystonin-a2

The following siRNA sequences were efficacious in knocking down dystonin-a1 and dystonin-a2 expression, respectively: Group A: Sense: 5’-ACAUGUACGUGGAGGAGCA-3’ / Antisense: 5’-UGCUCCUCCACGUACAUGU-3’; Sense: 5’-CAAGCAUGAGAGAUCCAAA-3’ / Antisense: 5’-UUUGGAUCUCUCAUGCUUG-3’; Group B: Sense: 5’-CAAGCAUGAGAGAUCCAAA-3’ […]