Miscellaneous - 14. page
NLSs
SV 40 Nucleoplasmin: AVKRPAATKKAGQAKKKKLD EGL-13: MSRRRKANPTKLSENAKKLAKEVEN c-Myc: PAAKRVKLD TUS-protein: KLKIKRPVK
Make a protein expression predominantly in membrane
A Kozak consensus sequence (CCAACCATGG) and Src myristoylation sequence (MGSSKSKPKDPSQR) are added to the N-terminal of ORF of specific gene.
Universal Primers
Standard primer name Sequence (5′->3′) Number of bases 1629DWN CTG TAA ATC AAC AAC GCA CAG 21 3AOX1 <GCA AAT GGC ATT CTG ACA TCC 21 5AOX1 < GAC TGG […]
Universal Primer List
PRIMERS SEQUENCES 3’ AD 5’ (AGA TGG TGC ACG ATG CAC AG) 3’ 3 AOX1 5′ (GCA AAT GGC ATT CTG ACA TCC) 3’ 5’ AD 5’ (TTC GAT […]
miRNA Database
http://www.mirbase.org/index.shtml
Multiple Sequence Alignment
http://www.ebi.ac.uk/Tools/msa/
Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy
Exosomes and microvesicles are extracellular nanovesicles released by most but not all cells. They are specifically equipped to mediate intercellular communication via the transfer of genetic information, including the transfer […]
Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells
Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived […]
Exosome
Exosomes are cell-derived vesicles that are present in many and perhaps all biological fluids, including blood, urine, and cultured medium of cell cultures. The reported diameter of exosomes is between […]