Summary of advantages and disadvantages of different techniques commonly used to transfect mammalian neurons Best suited for Strengths Limitations Toxicity Onset, level, and duration of expressiona Maximum insert size Genome […]
All posts by signagen - 29. page
Full Sequence of Ace2N-4AA-mNeon, A Novel Calcium Sensor
atggctgacgtggaaaccgagaccggcatgattgcacagtggattgtctttgctattatggctgctgctgctattgcttttggagtggctgtgcactttcggccttcagagctgaagagcgcatactatatcaac attgccatctgcactatcgccgctaccgcttactatgcaatggccgtgaactaccaggacctgacaatgaatggtgaaaggcaggtggtctacgcaagatatattaactgggtgctgaccacaccactgctcctg ctcgatctcatcgtcatgaccaagatgggcggagtgatgatttcttgggtcatcggcgcagacattttcatgatcgtgtttggtattctgggcgccttcgaggatgaacacaagttcaaatgggtgtactttatcg ctggatgtgtgatgcaggcagtcctgacatacgggatgtataacgccacttggaaagacgatctgaagaaaagccccgagtaccatagctcctatgtcagtctgctcgtcttcctgtcaatcctctgggtgtttt atcctgtcgtgtgggctttcgggtctggtagtggcgtgctgtccgtcgacaatgaggccattctcatgggaatcctggatgtgctcgctaagccactgtttggaatggggtgcctcattgcccatgagactatctt caagaagatgctgaggtctctcccagcgacacatgagttacacatctttggctccatcaacggtgtggactttgacatggtgggtcagggcaccggcaatccaaatgatggttatgaggagttaaacctgaagt ccaccaagggtgacctccagttctccccctggattctggtccctcatatcgggtatggcttccatcagtacctgccctaccctgacgggatgtcgcctttccaggccgccatggtagatggctccggataccaagtcc atcgcacaatgcagtttgaagatggtgcctcccttactgttaactaccgctacacctacgagggaagccacatcaaaggagaggcccaggtgaaggggactggtttccctgctgacggtcctgtgatgaccaact cgctgaccgctgcggactggtgcaggtcgaagaagacttaccccaacgacaaaaccatcatcagtacctttaagtggagttacaccactggaaatggcaagcgctacaggagcactgcgcggaccacctacac ctttgccaagccaatggcggctaactatctgaagaaccagccgatgtacgtgttccgtaagacggagctcaagcactccaagaccgagctcaacttcaaggagtggcaaaaggcctttaccgatgtgatgggcat ggacgagctgtacaagaagagcaggatcaccagcgagggcgagtacatccccctggaccagatcgacatcaacgtgttctgctacgagaacgaggtgtaatga
AAV–BDNF mediated attenuation of quinolinic acid-induced neuropathology and motor function impairment
Maintenance and plasticity of striatal neurons is dependant on brain-derived neurotrophic factor (BDNF), which is depleted in the Huntington’s disease striatum due to reduced expression and disrupted corticostriatal transportation. In […]
mIFP Full Sequence
atgtcggtaccgctgactacctcagcattcggccacgcgtttctggctaactgtgaacgcgagcagatccacctggcgggctccattcagccgcac ggtatcctgctggctgtgaaagagccggacaacgtggtgatccaggcttctattaacgctgcggagttcctgaacaccaactctgttgttggcc gtccgctgcgtgacctgggcggcgatctgcctttgcagatcctgccgcacctgaacggcccgctgcacctggctccgatgaccctgcgttgtacc gtgggttctccgccgcgtcgtgtggactgtaccattcatcgtccgtctaacggcggcctgatcgtagaactggaaccagcaaccaagaccact aacattgcgccggctctggacggtgcgtttcatcgtatcacttcttcatcctccctgatgggcctgtgtgacgaaaccgcgactattatccgtgaga ttactggctacgaccgtgtgatggtagtacgtttcgatgaagagggtaatggcgaaattctgtccgaacgtcgtcgtgcggacctggaagcgtt cctgggtaaccgctacccggcgtctactattccgcagatcgctcgtcgcctgtacgaacataaccgtgttcgcctgctggtagatgtgaactat actccggttccgctacagccgcgcatcagcccgctgaacggtcgtgatctggatatgtccctgtcttgcctgcgctctatgtccccgatccacca gaaatacatgcaggacatgggcgttggcgcgaccctggtttgctctctgatggtgtctggtcgtctgtggggtctgatcgcttgccaccacta cgaaccgcgcttcgttccgttccacattcgcgctgctggcgaagcgctggcggaaacttgtgcgatccgcatcgcgacgctggagagcttt gcacagtctcagtccaaatga
Infrared fluorescent proteins
Hunting for new fluorescent proteins in the coral reefs of the Caribbean and Australia is a task that a lucky few researchers have managed to get funding for. Scuba diving […]
Comparative Analysis of Cesium Chloride- and Iodixanol-Based Purification of Recombinant Adeno-Associated Viral Vectors for Preclinical Applications.
Cesium chloride (CsCl)- and iodixanol-based density gradients represent the core step in most protocols for serotype-independent adeno-associated virus (AAV) purification established to date. However, despite controversial reports about the purity […]
1st, 2nd Generations vs. Gutless Adenovirus Systems
Map of adenovirus serotype 5 genome and different generations of adenoviral vectors. Early transcripts are represented by E1–E4 regions and late transcripts are represented by L1–L5 regions. MLP: major late […]
NLSs
SV 40 Nucleoplasmin: AVKRPAATKKAGQAKKKKLD EGL-13: MSRRRKANPTKLSENAKKLAKEVEN c-Myc: PAAKRVKLD TUS-protein: KLKIKRPVK