CRISPR-mediated mouse genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and sgRNA into zygotes to achieve editing in one step. We developed a simple and economic electroporation-based strategy, designated as CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), to deliver Cas9/sgRNA ribonucleoproteins (RNPs) into mouse zygotes with 100% efficiency. CRISPR-EZ enables highly efficient and high-throughput genome editing in vivo, with a significant improvement in embryo viability compared with microinjection-based technology
Recent Posts
- AAV(cc47)-CMV-GFP is Ready to Package
- AAV(MYO)-CMV-GFP is Ready to Package
- AAV(REC2)-CMV-GFP is Ready to Package
- AAV Rh20 VP1 Gene
- AAV Rec2 Capsid Sequence
- Homemade qPCR Mix Recipe
- Home made qPCR Master Mix
- Nanopore Sequencing: Principles, Platforms and Advantages
- Nanopore Sequencing
- ssAAV9-CMV-SecNanoLuc & scAAV9-CMV-SecNanoLuc, Ready to Package
- AAV(BI30)-CAG-GFP-3xmiR122 is Ready to Package for the Transduction of the Endothelial Cells of CNS
- AAV(9-X1.1)-CMV-GFP is Ready to Package for the Transduction of the Endothelial Cells of CNS
- The Principle of Transformation
- IRES or 2A in my polycistronic expression cassette, which one is better?
- AAV[MyoAAV(2A)]-CMV-GFP and AAV[MyoAAV(4E)]-CMV-GFP Ready to Package
- How Orbital Diameter and Shaker Agitation Rate Affect Bacteria Growth
- scAAV(Ark313)-CBh-GFP Ready to Package for Optimal Mouse T Cell Transduction
- AAV(MG1.1)-CMV-GFP and AAV(MG1.2)-CMV-GFP Ready to Package for Microglia Transduction
- Gibson Assembly Cloning
- Tips for Maximizing Ligation Efficiencies
- Troubles with ligation?
- Electroporation Tips
- Electroporation vs. Heat Shock Transformation
- Causes and Solutions for Bad qPCR Efficiency
- Lentivirus Formulation Buffer
- A195 Buffer for Adenovirus Formulation and Long Term Storage
- DNA/RNA non-specific endonuclease [Serratia marcescens]
- DNA/RNA non-specific endonuclease [Serratia marcescens]
- DNA/RNA non-specific endonuclease [Serratia marcescens]
- Starting Materials to Run a ddPCR for rAAV Titration with An AutoDG Droplet Generator and QX 200 Droplet Reader
- Tips and tricks for performing ddPCR
- LV-EF1a-GFP-Puro in Baboon Envelope Pseudotype, Ready to Package
- Adenovirus Type C qPCR Titration Primers and Probe
- Density and Refractive Index for CsCl
- Cell Culture Antibiotic Selection Guide
- AAV5-RPhpe427-GFP and AAV5-RPhpe1289-GFP Ready to Package