LV-EF1α-Cre-Puro; LV-EF1A-Cre-Puro; LV-EF1α-Cre-IRES-Puro; EF1A-Cre-Puro Lentivirus; EF1α-Cre-Puro Lentivirus
Product Category: Pre-made recombinant lentivirus; LV-EF1α-iCre-Puro; LV-EF1A-iCre-Puro; LV-EF1α-iCre-IRES-Puro; EF1A-iCre-Puro Lentivirus;
EF1α-iCre-Puro Lentivirus
Description: Cre recombinase is a type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP
sites in
cells or experimental animals. By optimizing the mammalian codon usage for
Cre recombinase, Cre expression is improved in the mammalian cells. This codon-improved
Cre (known as iCre) transgene shows lower CpG content from the prokaryotic
coding sequence, thus reducing the chances of epigenetic silencing in mammals.
This
LV-EF1α-iCre-Puro
pre-made lentivirus expresses
iCre recombinase with
co-expression of puromycin selection in an IRES cassette under EF1α
promoter. Ready to use
format.
Titer: > 1x109 TU/mL
Selection Antibiotic: Puromycin
Purity*: In vivo grade and OK for both in vitro tissue culture infection and in vivo injection
Size: 2 x 25 µL (Std Pack)
Storage Buffer: PBS
Infection Protocol: 
Transfer Vector Map:
* Concentrated via PEG precipitation followed by ultra-centrifugation at 25000 rpm
