5′-GGACTTAAAGGCTCTTAAT-3′5′-GGACAAGAAAGCAGACAAA-3′
Human ChAT Promoter
cccgggagcagggggtggggaaggaggagcagtggctggtggcaagtctgagccaggacatagccgatgacagcctgtgaatgaccggccagactggggttggaccttcggggcagagcaggcagcccttggaaagccaggctggtaggagcatgggtgggaggcacaaaacacagacaaacagacatatatacacatatatcctctaggcatctccgaggagtgatggaggagagagttggtaggtgggaccacaggaaaccatcccacccagctctgccttcttcaagctcagggtccctgaagcctgactagtgtctcccgaatgggggaggggggggctctgggcagagcaaaaggaggcattggctgcatgtcttagggcctttaccaactgaccttgtccttgctgtcctctcaaaagactgccatgctccggtgaatcctcaccaggacgccacctctttcactttgcagttgggggcagcccctacgaacccacagcactgggaggcgtgggaacaagcctgtggagagtgtccctgcactgtgccagcaagggctttacattctctgcccagcactgcaaggcagccccaggctcctccatccttgggcctggttcatccatctttctggactctctgttcctggcaacaaccctgccagtcccgatacatgggcggtgcttgcctctgggtaggggtggtgttgggtggggagaggggtgaggaaattagctaaacctcagctttagacttgagagaatagagggtgggctggtggaaaacatgagtatgtgcctgtggaggcttaggttcagggggatctcagagggtagcaattgtgacccacagcctaataataacagtctttgccctcttggcctggctc
Validated Target Region against Mouse G3BP Gene
CCTCAGAGAGATCAGAGAGTT
Two Validated Mouse SV2C Target Regions
5′- GACAGCATCGTGTCTGTAG -3′5′- ATCGTGTCTGTAGGACAGC -3′
Validated Target Region against Mouse Cacna1c Gene
5′- GGAAAGCTCTATACCTGTT – 3′
Luna Universal Probe One-Step RT-qPCR for Detection of Covid-19
Reaction Setup: For best results, we recommend running each RNA standard and sample in triplicate. COMPONENT 20 µL REACTION FINAL CONCENTRATION Luna Universal Probe One-Step Reaction Mix (2X) 10 µL 1X […]
RT-qPCR Reaction Conditions for Detection of Covid-19 by FDA and CDC
In Stage 1, Set to 2 min at 25°C; 1 Rep.In Stage 2, Set to 15 min at 55°C; 1 Rep.In Stage 3, Set to 2 min at 95°C, 1 […]
Validated Target Region against Mouse PTBP1 Gene
5′-GGGTGAAGATCCTGTTCAATA-3′
Research Use Only 2019-Novel Coronavirus (2019-nCoV, Covid-19) Real-time RT-PCR Primers and Probes
Name Description Oligonucleotide Sequence (5’>3’) Label1 FinalConc. 2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer GAC CCC AAA ATC AGC GAA AT None 500nM 2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer TCT GGT TAC TGC CAG TTG […]