AAV Serotype Capsid Mutation(s) AAV2 Y730F AAV2 Y444F AAV2 Y444F+Y730F AAV2 Y444F+Y730F+Y500F AAV2 Y444F+Y730F+Y500F+Y272F AAV8 Y733F AAV8 Y447F AAV9 Y446F AAV9 Y731F AAV9 Y446F + Y731F
Adeno-associated Virus (AAV) - 18. page
AAV 2 vector: Point mutations in tyrosines lead to high-efficiency transduction.
AAV2-mediated transduction of hepatocytes from normal C57BL/6 mice injected via tail vein with tyrosine-mutant capsid scAAV2-EGFP vectors. (A) Transgene expression was detected by fluorescence microscopy 2 weeks after injection of […]
Suppression of microRNA via Tough Decoy rAAV
http://signagen.com/Application_Notes/Application_Note_AAV_2015_9.pdf
The WPRE reduces readthrough transcription from retroviral vectors
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed […]
What is WPRE?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]
Systematic Comparison of Constitutive Promoters and the Doxycycline-Inducible Promoter
Constitutive promoters are used routinely to drive ectopic gene expression. Here, we carried out a systematic comparison of eight commonly used constitutive promoters (SV40, CMV, UBC, EF1A, PGK and CAGG […]
Comparison of EF-1 alpha and CMV promoterd for engineering stable tumor cell lines using rAAV
Stable HT-29 clones with > 97% of all cells homogeneously expressing GFP were generated with the EF-1 alpha promoter. In contrast in clones carrying the CMV promoter, only up to […]
Why EF1alpha promoter is so special?
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. […]
Plasmids for Optogenetics Sensors from Addgene
Addgene’s repository contains a variety of genetically encoded biosensors to monitor and measure fluctuations in molecular signals in cells, including those to monitor calcium, glutamate, pH, and voltage. These optogenetics […]