Most current methods of gene delivery for primary cultured hippocampal neurons are limited by toxicity, transient expression, the use of immature neurons, and/or low efficiency. We performed a direct comparison […]
Adeno-associated Virus (AAV) - 15. page
Differential Effects of AAV-BDNF and AAV-NTF3 in the Deafened Adult Guinea Pig Ear
Intracochlear neomycin administration resulted in a complete loss of hair cells and supporting cells in the basal turn, forming a flat epithelium. A few looping auditory fibers are seen in […]
A Novel Synthetic AAV Capsid 8BP2 for Efficient Transduction of Retinal Bipolar Cells
The map of AAV serotype 8BP2
Full Sequence of Ace2N-4AA-mNeon, A Novel Calcium Sensor
atggctgacgtggaaaccgagaccggcatgattgcacagtggattgtctttgctattatggctgctgctgctattgcttttggagtggctgtgcactttcggccttcagagctgaagagcgcatactatatcaac attgccatctgcactatcgccgctaccgcttactatgcaatggccgtgaactaccaggacctgacaatgaatggtgaaaggcaggtggtctacgcaagatatattaactgggtgctgaccacaccactgctcctg ctcgatctcatcgtcatgaccaagatgggcggagtgatgatttcttgggtcatcggcgcagacattttcatgatcgtgtttggtattctgggcgccttcgaggatgaacacaagttcaaatgggtgtactttatcg ctggatgtgtgatgcaggcagtcctgacatacgggatgtataacgccacttggaaagacgatctgaagaaaagccccgagtaccatagctcctatgtcagtctgctcgtcttcctgtcaatcctctgggtgtttt atcctgtcgtgtgggctttcgggtctggtagtggcgtgctgtccgtcgacaatgaggccattctcatgggaatcctggatgtgctcgctaagccactgtttggaatggggtgcctcattgcccatgagactatctt caagaagatgctgaggtctctcccagcgacacatgagttacacatctttggctccatcaacggtgtggactttgacatggtgggtcagggcaccggcaatccaaatgatggttatgaggagttaaacctgaagt ccaccaagggtgacctccagttctccccctggattctggtccctcatatcgggtatggcttccatcagtacctgccctaccctgacgggatgtcgcctttccaggccgccatggtagatggctccggataccaagtcc atcgcacaatgcagtttgaagatggtgcctcccttactgttaactaccgctacacctacgagggaagccacatcaaaggagaggcccaggtgaaggggactggtttccctgctgacggtcctgtgatgaccaact cgctgaccgctgcggactggtgcaggtcgaagaagacttaccccaacgacaaaaccatcatcagtacctttaagtggagttacaccactggaaatggcaagcgctacaggagcactgcgcggaccacctacac ctttgccaagccaatggcggctaactatctgaagaaccagccgatgtacgtgttccgtaagacggagctcaagcactccaagaccgagctcaacttcaaggagtggcaaaaggcctttaccgatgtgatgggcat ggacgagctgtacaagaagagcaggatcaccagcgagggcgagtacatccccctggaccagatcgacatcaacgtgttctgctacgagaacgaggtgtaatga
AAV–BDNF mediated attenuation of quinolinic acid-induced neuropathology and motor function impairment
Maintenance and plasticity of striatal neurons is dependant on brain-derived neurotrophic factor (BDNF), which is depleted in the Huntington’s disease striatum due to reduced expression and disrupted corticostriatal transportation. In […]
Comparative Analysis of Cesium Chloride- and Iodixanol-Based Purification of Recombinant Adeno-Associated Viral Vectors for Preclinical Applications.
Cesium chloride (CsCl)- and iodixanol-based density gradients represent the core step in most protocols for serotype-independent adeno-associated virus (AAV) purification established to date. However, despite controversial reports about the purity […]
Efficient transduction and optogenetic stimulation of retinal bipolar cells by a synthetic adeno‐associated virus capsid and promoter
Analysis of transduction properties of AAV2/8BP2 virus A, B Representative 20× confocal images of immunostained vibratome sections from retinas of mice subretinally injected (A) or intravitreally injected (B) with viruses. […]
ssAAV vs. scAAV
For ssAAV vectors, the coding sequence and complementary sequence of the transgene expression cassette are on separate strands and are packaged in separate viral capsids. For scAAV vectors, both the […]