miRBase provides the following services: The miRBase database is a searchable database of published miRNA sequences and annotation. Each entry in the miRBase Sequence database represents a predicted hairpin portion […]
Miscellaneous - 16. page
How to locate promoter sequence for a specific gene
Many people have problem identifying or predicting the promoter sequence of a gene, or don’t know how to get the actual sequence for analysis such as primer design, transcription factor […]
List of Protein Tags
Peptide tags AviTag, a peptide allowing biotinylation by the enzyme BirA and so the protein can be isolated by streptavidin (GLNDIFEAQKIEWHE) Calmodulin-tag, a peptide bound by the protein calmodulin (KRRWKKNFIAVSAANRFKKISSSGAL) […]
Alternatives to Fetal Bovine Serum in Cell Culture media, Challenges and Perspectives
Dr. Gerhard Gstraunthaler recently presented a seminar with the above title. Gerhard carries a faculty appointment with the Department of Physiology at Innsbruck Medical University, Austria. Gerhard is an advocate of serum-free […]
Role of Serum in Cell Culture Media
Serum provides > growth factors and hormones > binding and transport proteins > attachment and spreading factors > additional amino acids, vitamins and trace elements > fatty acids and lipids > […]
Fetal Bovine Serum – the Tipping Point
Users of US Fetal Bovine Serum have been watching for months and months how prices climb up along with a constantly growing shortage and with no end in sight. Yet […]
Fetal Bovine Serum Availability – How do Regulations Affect It?
Now we will talk about the influence of various regulations on the availability of Fetal Bovine Serum. It is a fact that import and export regulations have direct influence on […]
Real Time PCR Dyes
Dye Structure Color Vendor Cat # Price Features SYBR Green 1 Green unstable at 4 °C SYTO-82 Orange does not inhibit PCR, show no preferential binding to GC rich sequences […]
Fusion Cloning, Phusion Cloning, Pfusion Cloning and Confusion PCR Cloning
In-Fusion PCR Cloning: Clontech, http://www.freepatentsonline.com/7575860.html Cold Fusion Cloning Kit: SBI Fast Seamless Cloning Kit: Dogene CloneEZ Kit: Genescript GENEART Seamless Cloning and Assembly Kit: Invitrogen SLIC uses the 3′ […]
Gibson Assembly Mix for joining two or more PCR fragments
1) Prepare 6 ml of 5X ISO Buffer in a 15 ml falcon tube as follows: 3 ml 1 M Tris-HCl pH 7.5 + 150µl 2 M MgCl2 + 240 µl 100 […]