Summary of advantages and disadvantages of different techniques commonly used to transfect mammalian neurons Best suited for Strengths Limitations Toxicity Onset, level, and duration of expressiona Maximum insert size Genome […]
Miscellaneous - 14. page
mIFP Full Sequence
atgtcggtaccgctgactacctcagcattcggccacgcgtttctggctaactgtgaacgcgagcagatccacctggcgggctccattcagccgcac ggtatcctgctggctgtgaaagagccggacaacgtggtgatccaggcttctattaacgctgcggagttcctgaacaccaactctgttgttggcc gtccgctgcgtgacctgggcggcgatctgcctttgcagatcctgccgcacctgaacggcccgctgcacctggctccgatgaccctgcgttgtacc gtgggttctccgccgcgtcgtgtggactgtaccattcatcgtccgtctaacggcggcctgatcgtagaactggaaccagcaaccaagaccact aacattgcgccggctctggacggtgcgtttcatcgtatcacttcttcatcctccctgatgggcctgtgtgacgaaaccgcgactattatccgtgaga ttactggctacgaccgtgtgatggtagtacgtttcgatgaagagggtaatggcgaaattctgtccgaacgtcgtcgtgcggacctggaagcgtt cctgggtaaccgctacccggcgtctactattccgcagatcgctcgtcgcctgtacgaacataaccgtgttcgcctgctggtagatgtgaactat actccggttccgctacagccgcgcatcagcccgctgaacggtcgtgatctggatatgtccctgtcttgcctgcgctctatgtccccgatccacca gaaatacatgcaggacatgggcgttggcgcgaccctggtttgctctctgatggtgtctggtcgtctgtggggtctgatcgcttgccaccacta cgaaccgcgcttcgttccgttccacattcgcgctgctggcgaagcgctggcggaaacttgtgcgatccgcatcgcgacgctggagagcttt gcacagtctcagtccaaatga
Infrared fluorescent proteins
Hunting for new fluorescent proteins in the coral reefs of the Caribbean and Australia is a task that a lucky few researchers have managed to get funding for. Scuba diving […]
NLSs
SV 40 Nucleoplasmin: AVKRPAATKKAGQAKKKKLD EGL-13: MSRRRKANPTKLSENAKKLAKEVEN c-Myc: PAAKRVKLD TUS-protein: KLKIKRPVK
Make a protein expression predominantly in membrane
A Kozak consensus sequence (CCAACCATGG) and Src myristoylation sequence (MGSSKSKPKDPSQR) are added to the N-terminal of ORF of specific gene.
Universal Primers
Standard primer name Sequence (5′->3′) Number of bases 1629DWN CTG TAA ATC AAC AAC GCA CAG 21 3AOX1 <GCA AAT GGC ATT CTG ACA TCC 21 5AOX1 < GAC TGG […]
Universal Primer List
PRIMERS SEQUENCES 3’ AD 5’ (AGA TGG TGC ACG ATG CAC AG) 3’ 3 AOX1 5′ (GCA AAT GGC ATT CTG ACA TCC) 3’ 5’ AD 5’ (TTC GAT […]
miRNA Database
http://www.mirbase.org/index.shtml
Multiple Sequence Alignment
http://www.ebi.ac.uk/Tools/msa/