ACGCGTAACTTGTGGACTAAGTTTGTTCACATCCCCTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCA CACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAGGTGTTGGGGAGGCAGTTACCGGG GCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGCGAGGCTGTGAGCAGCCACAGTGCCCTGCTC AGAAGCCCCAAGCTCGTCAGTCAAGCCGGTTCTCCGTTTGCACTCAGGAGCACGGGCAGGCGAGTGGCCCCTAGTTCTGGGGGCAGCTCTAGAG CCGATCCCCGGGTACCGGT
Miscellaneous - 10. page
Secreted Gaussia Luciferase ORF
ATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGGCCAAGCCCACCGAGAACAACGAAGACTTCAACATCGTGGCCGTGGCCAG CAACTTCGCGACCACGGATCTCGATGCTGACCGCGGGAAGTTGCCCGGCAAGAAGCTGCCGCTGGAGGTGCTCAAAGAGATGGAAGCCAATGCCCGG AAAGCTGGCTGCACCAGGGGCTGTCTGATCTGCCTGTCCCACATCAAGTGCACGCCCAAGATGAAGAAGTTCATCCCAGGACGCTGCCACACCTACGA AGGCGACAAAGAGTCCGCACAGGGCGGCATAGGCGAGGCGATCGTCGACATTCCTGAGATTCCTGGGTTCAAGGACTTGGAGCCCATGGAGCAGTTCA TCGCACAGGTCGATCTGTGTGTGGACTGCACAACTGGCTGCCTCAAAGGGCTTGCCAACGTGCAGTGTTCTGACCTGCTCAAGAAGTGGCTGCCGCAAC GCTGTGCGACCTTTGCCAGCAAGATCCAGGGCCAGGTGGACAAGATCAAGGGGGCCGGTGGTGACTAA
NCOR1 and MSTN Validated Target Region Sequence
NCOR1 target: GCTCTCAAAGTTCAGACTCTT MSTN target: GATTGGATTATCGCTCCTAAA
gpNa V 1.7-targeting shRNA
5′-GGGCATAGATTACGTGAAA TTCAAGAGA TTTCACGTAATCTATGCCC-3′
Cas9 Species/Variants and PAM Sequences
Species/Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 NGG SpCas9 D1135E variant NGG (reduced NAG binding) SpCas9 VRER variant NGCG SpCas9 EQR variant NGAG SpCas9 VQR variant NGAN or […]
Codon optimized PEST sequence
5′-TCC CAC GGC TTC CCC CCC GAG GTC GAG GAG CAG GCC GCC GGC ACC CTC CCC ATG AGC TGC GCC CAG GAG AGC GGC ATG GAC AGG CAC CCC […]
pTRE-miniCMV or CMVtight
316 bp: gagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctat cagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaa cgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcc
Human GPR55 siRNA
Human GPR55 siRNAs: 5′-GAAUUCCGCAUGAACAUCAUU-3′ 5′-GAGAAACAGCUUUAUCGUAUU-3′ 5′-AAGAACAGGUGGCCCGAUUUU-3′ 5′-GCUACUACUUUGUCAUCAAUU-3′
Several Published siRNAs
iRNA sequences JAK1 #1 CCACAUAGCUGAUCUGAAA JAK1 #2 GACAUGAUAUUGAGAACGA JAK1 #3 UUACAAGGAUGACGAAGGA JAK1 #4 CGGAUGAGGUUCUAUUUCA JAK2 SP GAGCAAAGAUCCAAGACUA GCCAGAAACUUGAAACUUA GAUCCUGGCAUUAGUAUUA ACAGAAUGCUGGAACAAUA TYK2 SP GCAUCCACAUUGCACAUAA UCAAAUACCUAGCCACACU CAAUCUUGCUGACGUCUUG CACAAGGACCAACGUGUAU JAK3 SP GCGCCUAUCUUUCUCCUUU CCAGAAAUCGUAGACAUUA […]
A Validated siRNA Targeting Mouse/Rat RGS4/5
RGS5 : CAGACUCUGCUGUUGACCUUGUCAU GGUGAACAUUGACCACUUCACUAAA RGS4: AAAGCUGCCAGUCCACAUUCAUGGU