Miscellaneous - 10. page

Human FOXO1 siRNAs: 5′-GGAGGUAUGAGUCAGUAUATT-3′ (sense) 5 ′-UAUACUGACUCAUACCUCCTT-3 ′ (antisense),  

Human Period 2 (hPer2) target sequences were as follows: i) 5’-CAACAAGGTGCTGAGAGTCAGCTTT-3’ ii) 5’-ACTCTGGTTATGAAGCCCCTAGAAT-3’

Two validated Gadd45a siRNAs siRNA1: sense: 5′- GAG CAG AAG ACC GAA AGG AUG GAU A-3′; anti-sense: 5′-UAU CCA UCC UUU CGG UCU UCU GCU C-3′ siRNA2: sense 5′-GGU GAC […]

ACGCGTAACTTGTGGACTAAGTTTGTTCACATCCCCTTCTCCAACCCCCTCAGTACATCACCCTGGGGGAACAGGGTCCACTTGCTCCTGGGCCCA CACAGTCCTGCAGTATTGTGTATATAAGGCCAGGGCAAAGAGGAGCAGGTTTTAAAGTGAAAGGCAGGCAGGTGTTGGGGAGGCAGTTACCGGG GCAACGGGAACAGGGCGTTTCGGAGGTGGTTGCCATGGGGACCTGGATGCTGACGAAGGCTCGCGAGGCTGTGAGCAGCCACAGTGCCCTGCTC AGAAGCCCCAAGCTCGTCAGTCAAGCCGGTTCTCCGTTTGCACTCAGGAGCACGGGCAGGCGAGTGGCCCCTAGTTCTGGGGGCAGCTCTAGAG CCGATCCCCGGGTACCGGT

ATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGGCCAAGCCCACCGAGAACAACGAAGACTTCAACATCGTGGCCGTGGCCAG CAACTTCGCGACCACGGATCTCGATGCTGACCGCGGGAAGTTGCCCGGCAAGAAGCTGCCGCTGGAGGTGCTCAAAGAGATGGAAGCCAATGCCCGG AAAGCTGGCTGCACCAGGGGCTGTCTGATCTGCCTGTCCCACATCAAGTGCACGCCCAAGATGAAGAAGTTCATCCCAGGACGCTGCCACACCTACGA AGGCGACAAAGAGTCCGCACAGGGCGGCATAGGCGAGGCGATCGTCGACATTCCTGAGATTCCTGGGTTCAAGGACTTGGAGCCCATGGAGCAGTTCA TCGCACAGGTCGATCTGTGTGTGGACTGCACAACTGGCTGCCTCAAAGGGCTTGCCAACGTGCAGTGTTCTGACCTGCTCAAGAAGTGGCTGCCGCAAC GCTGTGCGACCTTTGCCAGCAAGATCCAGGGCCAGGTGGACAAGATCAAGGGGGCCGGTGGTGACTAA

NCOR1 target: GCTCTCAAAGTTCAGACTCTT MSTN target: GATTGGATTATCGCTCCTAAA

5′-GGGCATAGATTACGTGAAA  TTCAAGAGA TTTCACGTAATCTATGCCC-3′

Species/Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 NGG SpCas9 D1135E variant NGG (reduced NAG binding) SpCas9 VRER variant NGCG SpCas9 EQR variant NGAG SpCas9 VQR variant NGAN or […]

5′-TCC CAC GGC TTC CCC CCC GAG GTC GAG GAG CAG GCC GCC GGC ACC CTC CCC ATG AGC TGC GCC CAG GAG AGC GGC ATG GAC AGG CAC CCC […]

316 bp: gagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctat cagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaa cgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcc