HEK293 cells can produce over 100,000 adenovirus particles per cell when using optimized protocols and transfection methods. Here’s a more detailed explanation:
Adenovirus
How to Amplify An Adenovirus
To amplify an adenovirus, you typically infect a permissive cell line, like HEK 293 cells, with the adenovirus at a suitable multiplicity of infection (MOI), allowing the virus to replicate […]
A195 Buffer for Adenovirus Formulation and Long Term Storage
10 mM Tris-HCl, 10 mM histidine, 75 mM NaCl, 5% sucrose (w/v), 1 mM MgCl2, 0.02% (w/v) PS-80 (polysorbate-80), 0.1 mM EDTA, 0.5% Ethanol (v/v), pH 7.4
Adenovirus Type C qPCR Titration Primers and Probe
Target Penton:FORWARD: TCGACACCACCCGTGTGTACREVERSE: TGCTGTGGTCGTTCTGGTAGTTPROBE: TGGACAACAAGTCAACGGATGTGGCA
1st, 2nd Generations vs. Gutless Adenovirus Systems
Map of adenovirus serotype 5 genome and different generations of adenoviral vectors. Early transcripts are represented by E1–E4 regions and late transcripts are represented by L1–L5 regions. MLP: major late […]
The WPRE reduces readthrough transcription from retroviral vectors
The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) increases transgene expression from a variety of viral vectors, although the precise mechanism is not known. WPRE is most effective when placed […]
What is WPRE?
Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) is a DNA sequence that, when transcribed creates a tertiary structure enhancing expression. Commonly used in molecular biology to increase expression of […]
Key properties of a viral vector
Viral vectors are tailored to their specific applications but generally share a few key properties: Safety: Although viral vectors are occasionally created from pathogenic viruses, they are modified in such […]
SignaGen’s Ad.MAX™ Adenovirus Expression System for Maximum Adenovirus Production
Ad.MAX™ Technology for Maximum Adenovirus Production: Ad.MAX™ technology was developed by genetically modifying virus packaging cell, HEK293 cell and adenoviral shuttle vector or adenoviral genome for maximum adenovirus production. The […]