As DNA is a highly hydrophilic molecule, it usually cannot pass through the cell membrane of bacteria. Hence, to make bacteria capable of internalizing genetic material, they must be made […]
Monthly Archives: July 2023
IRES or 2A in my polycistronic expression cassette, which one is better?
When designing a gene expression vector to co-express multiple ORFs under the control of a single promoter, you can choose to place multiple ORFs behind the promoter, separated by linkers […]